alexa Optimization of RAPD-PCR Protocol to Screen Jatropha Cu
ISSN:2157-7463

Journal of Petroleum & Environmental Biotechnology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Optimization of RAPD-PCR Protocol to Screen Jatropha Curcas and Gossypium Hirsutum Grown in Metal Contaminated Soil

Narayanan Mathiyazhagan and Devarajan Natarajan*
Department of Biotechnology, Periyar University, Salem 636 011, Tamil Nadu, India
Corresponding Author : Narayanan Mathiyazhagan
Research scholar, Department of Biotechnology
Periyar University, Salem-11, Tamil Nadu, India
Tel: +91 9486502785
E-mail: [email protected]
Received July 15, 2011; Accepted December 10, 2011; Published December 12, 2011
Citation: Mathiyazhagan N, Natarajan D (2012) Optimization of RAPD-PCR Protocol to Screen jpebropha Curcas and Gossypium Hirsutum Grown in Metal Contaminated Soil. J Phylogenetics Evol Biol 3:113. doi:10.4172/2157-7463.1000113
Copyright: © 2012 Mathiyazhagan N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

The optimization of randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) protocol was performed to screen two plants ( Jatropha curcas & Gossypium hirsutum ) grown in metal contaminated soils. A CTAB method was employed for DNA extraction, but an overnight RNase treatment was carried out to eliminate RNA contaminants. The isolated DNA was used for RAPD-PCR amplification. The optimum condition for reliable amplification requires a higher concentration of MgCl 2 (3 mM), primer (2.5 μM), Taq DNA polymerase (1 unit) and 3μl of template DNA (sample) and an annealing temperature of 55°C. Reproducible amplified products were observed in these conditions for both the plant species.

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords