alexa Phylogenetic Grouping of Dominant Fecal Escherichia coli Isolates from Healthy Males and Females in Al-Kut/Wasit Province/Iraq | OMICS International | Abstract
ISSN: 2155-9597

Journal of Bacteriology & Parasitology
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Research Article

Phylogenetic Grouping of Dominant Fecal Escherichia coli Isolates from Healthy Males and Females in Al-Kut/Wasit Province/Iraq

Sareaa MG Al-Mayahie1*, Alaa AM Al-Khafajy1, Nuha AS Dosh2, Assel RK Al-Rekabi1 and Ahlam GN Al-Atabie1

1Department of Biology, College of Science, University of Wasit, Wasit, Iraq

2Department of Microbiology, College of Medicine, University of Kufa, Kufa, Iraq

*Corresponding Author:
Sareaa MG Al-Mayahie
Department of Biology, College of Science
University of Wasit, Wasit 52004, Iraq
Tel: 096407802833209
E-mail: [email protected]

Received date: December 07, 2014; Accepted date: January 28, 2015; Published date: February 02, 2015

Citation: Al-Mayahie SMG, Al-Khafajy AAM, Dosh NAS, Al-Rekabi ARK, Al-Atabie AGN (2015) Phylogenetic Grouping of Dominant Fecal Escherichia coli Isolates from Healthy Males and Females in Al-Kut/Wasit Province/Iraq. J Bacteriol Parasitol 6:215. doi: 10.4172/2155-9597.1000215

Copyright: © 2015 Sareaa MG Al-Mayahie. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Fecal isolates of Escherichia coli are divided into four main phylogenetic groups designated A, B1, B2 and D. Most commensal strains belong to group A and have fewer virulence factors than the extra intestinal pathogenic E. coli strains (ExPEC). This study was mainly designed for phylogenetic grouping of commensal fecal E. coli isolates from healthy males and females in Al-Kut City/Wasit Province/Iraq using PCR-based protocols. Also ExPEC's virulence genes were detected among these isolates. Among this study included isolates (n=205), group A was the most common among both females' and males' isolates (68.5% vs. 63.0%, respectively), followed by groups: B1 (15.7% vs. 22.6%, respectively), D (10.7% vs. 9.5%, respectively), and B2 (4.1% vs. 4.7%, respectively). Gender distribution of phylogenetic groups showed insignificant differences between females and males. Females' and males' isolates did not differ significantly for all of the detected ExPEC's virulence genes, except for papC which was significantly (P ≤ 0.05) more prevalent among males' isolates (16.6%) than among females' isolates (6.6%). In both females' and males' isolates the most prevalent virulence genes were fimH (97.5% vs. 100%, respectively) and iucC (52.0% vs. 55.9%, respectively), whereas the least prevalent were sfa/foc (0% each) and hly (0.82% vs. 0%, respectively). Furthermore, virulence genes were concentrated in isolates clustered in group B2.
In both females and males, high percent of dominant commensal fecal E. coli isolates from Iraqi people in Al-Kut City clustered in phylogroup A followed by groups B1 and D whereas group B2 was rare. Also, dominant fecal strains with ExPEC characteristics were much less prevalent.

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