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ISSN: 2157-7439

Journal of Nanomedicine & Nanotechnology
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Research Article

Plant In vitro Culture goes Nano: Nanosilver-Mediated Decontamination of Ex vitro Explants

Nasser Mahna1*, Sepideh Zununi Vahed2,3,4 and Sajjad Khani2,3

1Department of Horticultural Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

2Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

3School of Advanced Biomedical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

4Faculty of Biology, Tarbiat Moallem Azarbayjan University, Tabriz, Iran

*Corresponding Author:
Nasser Mahna
Department of Horticultural Sciences
Faculty of Agriculture
University of Tabriz, Tabriz, Iran
Tel: 98-411-3392027
Fax: +98-411-3345332
E-mail: [email protected]

Received Date: December 12, 2012; Accepted Date: February 01, 2013; Published Date: February 04, 2013

Citation: Mahna N, Vahed SZ, Khani S (2013) Plant In vitro Culture goes Nano: Nanosilver-Mediated Decontamination of Ex vitro Explants. J Nanomed Nanotechol 4:161. doi:10.4172/2157-7439.1000161

Copyright: © 2013 Mahna N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Plant tissue culture is a basic and fundamental component of plant biotechnology, and progress in different fields of biotechnology greatly depends on the improvement of this technique. Nowadays, nanomaterials especially Nanosilver [NS], are regularly being used as an antimicrobial agent in medical and environmental fields. In order to assay the efficiency of NS in sterilizing plant seeds and fragile tissues, such as leaf and cotyledon, two important model plants, Arabidopsis and tomato, as well as potato as an important cultivated crop, were used as explants in this study. Briefly, samples soaked in different concentrations of NS with various exposure times, and then transferred onto the MS medium. Results showed that at lower concentrations, NS could function as an antimicrobial agent with no side effect on the explant viability, and consequently, all decontaminated seeds germinated, and leaf and cotyledon explants survived.

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