Potentiometric Sensor for Determination of Tramadol Hydrochloride in Pharmaceutical Preparations and Biological Fluids
- *Corresponding Author:
- Dr. Hazem M. Abu Shawish
College of Sciences
Al-Aqsa University, Gaza, Palestine
E-mail: [email protected]
Received date: August 25, 2010; Accepted date: September 26, 2010; Published date: September 28, 2010
Citation: Abu Shawish HM, Al-Dalou AR, Abu Ghalwa N, Abou Assi AA (2010) Potentiometric Sensor for Determination of Tramadol Hydrochloride in Pharmaceutical Preparations and Biological Fluids. Pharm Anal Acta 1:103. doi: 10.4172/2153-2435.1000103
Copyright: © 2010 Abu Shawish HM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A potentiometric tramadol-selective electrode based on the ion-association of tramadol hydrochloride (TDCl) with phosphomolybdic acid (TD-PM) is developed. The electrode exhibits a Nernstian slope of 58.3±0.7 mV/decade for tramadol ions in the concentration range 2.0 × 10 -6 - 1.0 × 10 -1 M with the limit of detection of 1.3 × 10 -6 M. The electrode has a fast and stable response time 5-8 s, good reproducibility and it can be used in pH range of 1.8-6.1. The present electrode show good discrimination of tramadol hydrochloride from several inorganic, organic ions, sugars and some common drug excipients. These characteristics of the electrode enable it to be used successfully for determination of tramadol hydrochloride in its pharmaceutical preparations and biological fl uids (urine and milk).