alexa Pterocarpus marsupium Derived Phyto-Synthesis of Copper
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
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Research Article

Pterocarpus marsupium Derived Phyto-Synthesis of Copper Oxide Nanoparticles and their Antimicrobial Activities

Rajgovind1, Gaurav Sharma1, Deepak Gupta Kr3, Nakuleshwar Dut Jasuja1* and Suresh Joshi C2

1School of Sciences, Suresh Gyan Vihar University, Rajasthan, India

2Department of Zoology, University of Rajasthan, Jaipur, India

3Centre for Converging Technologies, University of Rajasthan, Jaipur, India

*Corresponding Author:
Nakuleshwar Dut Jasuja
School of Sciences, Suresh Gyan
Vihar University, Jaipur, Rajasthan, India
Tel: +91-9414658277
E-mail: [email protected]

Received Date: April 03, 2015; Accepted Date: April 22, 2015; Published Date: April 29, 2015

Citation: Rajgovind, Sharma G, Deepak Gupta Kr, Jasuja ND, Suresh Joshi C (2015) Pterocarpus marsupium Derived Phyto-Synthesis of Copper Oxide Nanoparticles and their Antimicrobial Activities. J Microb Biochem Technol 7:140-144. doi:10.4172/1948-5948.1000195

Copyright: © 2015 Rajgovind, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

 

Abstract

In present study, copper oxide nanoparticles (CuONPs) synthesized by quick and eco-friendly phytogenicreduction of copper salt (copper sulphate CuSO4.H2O) solution with Pterocarpus marsupium extract. UV-VIS spectrometry indicated formation of nanoparticles via absorption spectra of copper colloidal solution at 442 nm. Phytosynthesis of CuONPs were further characterized by Transmission electron microscopy; scanning electron microscopy and Fourier transform infrared spectroscopy. The experimental results showed that diameter of CuONPs in colloidal solution were < 40 nm. Further, antibacterial activities of CuONPs were determined against Gram negative Escherichia coli- MTCC-9721, Proteus vulgaris- MTCC-7299, Klebsiella pneumonia- MTCC-9751 and Gram positive i.e. Staphylococcus aureus- MTCC-9442, Staphylococcus. epidermidis- MTCC- 2639, Bacillus cereus- MTCC-9017 bacteria by well agar diffusion and microdilution method. Notably, The CuONPs showed an effective antibacterial activity against all test microorganisms where K. pneumonia and E.coli showed maximum ZOI and MIC respectively i.e. 24 mm and 6 μg/ml.

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