alexa Rapid and Sensitive Determination Of Montelukast in Human Plasma by High Performance Liquid Chromatographic Method Using Monolithic Column: Application to Pharmacokinetic Studies
ISSN: 0975-0851

Journal of Bioequivalence & Bioavailability
Open Access

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Research Article

Rapid and Sensitive Determination Of Montelukast in Human Plasma by High Performance Liquid Chromatographic Method Using Monolithic Column: Application to Pharmacokinetic Studies

Alireza Shafaati1*, Afshin Zarghi1, Seyed Mohsen Foroutan1, Arash Khoddam2 and Babak Madadian2

1School of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, P.O.Box: 14155-6153, Iran

2Noor Research and Educational Institute, Tehran, Iran

*Corresponding Author:
Dr. Alireza Shafaati
Professsor of pharmaceutical chemistry
School of Pharmacy
Shaheed beheshti University of Medical Sciences
Tehran, P.O.Box: 14155-6153, Iran
E-mail: [email protected]

Received Date: May 24, 2010; Accepted Date: November 22, 2010; Published Date: January 11, 2011

Citation: Shafaati A, Zarghi A, Foroutan SM, Khoddam A, Madadian B (2010) Rapid and Sensitive Determination Of Montelukast in Human Plasma by High Performance Liquid Chromatographic Method Using Monolithic Column: Application to Pharmacokinetic Studies. J Bioequiv Availab 2: 135-138. doi: 10.4172/jbb.1000046

Copyright: © 2010 Shafaati A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

Abstract

A rapid, simple and sensitive high-performance liquid chromatography (HPLC) method, using a monolithic column and fluorescence detection, has been developed for quantification of montelukast in human plasma. Ethoxyquine, easily available as a pharmaceutical substance, was selected as internal satandard. The assay enables the measurement of montelukast for therapeutic drug monitoring with a minimum detectable limit of 5 ng/ ml−1. The method involves simple, one-step extraction procedure and analytical recovery was about 97%. The separation was carried out in reversed- phase conditions using a Chromolith RP® (RP-18e, 100 mm×4.6 mm) column at ambient temperature. The mobile phase was 56% acetonitrile and 50mM sodium dihydrogen phosphate, and distilled water to 100%, adjusted to pH 7.0 at a flow rate of 2 ml/min. The excitation wavelength was set at 350 nm, emission at 450 nm. The calibration curve was linear over the concentration range 20–800 ng/ml. The coefficients of variation for inter-day and intra-day assay were found to be less than 7%. The method was applied to the determination of montelukast in plasma from 12 subjects dosed with montelukast 10 mg tablets and pharmacokinetic parameters were determined.

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