alexa Simultaneous Determination of Salmeterol Xinafoate and Fluticasone Propionate in Bulk Powder and SeritideDiskus using High Performance Liquid Chromatographic and Spectrophotometric Method
ISSN : 2153-2435

Pharmaceutica Analytica Acta
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Research Article

Simultaneous Determination of Salmeterol Xinafoate and Fluticasone Propionate in Bulk Powder and SeritideDiskus using High Performance Liquid Chromatographic and Spectrophotometric Method

Ahmed Samir1, Hesham Salem1* and Mohammad Abdelkawy2

1Analytical Chemistry Department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA University), October 6 City, Egypt

2Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Egypt

*Corresponding Author:
Hesham Salem
Analytical Chemistry Department, Faculty of Pharmacy
October University for Modern Sciences and Arts (MSA University)
October 6 City, Egypt
Tel:
+2-100-60-500-29
E-mail: [email protected]

Received date: March 03, 2012; Accepted date: October 27, 2012; Published date: October 29, 2012

Citation: Samir A, Salem H, Abdelkawy M (2012) Simultaneous Determination of Salmeterol Xinafoate and Fluticasone Propionate in Bulk Powder and Seritide® Diskus using High Performance Liquid Chromatographic and Spectrophotometric Method. Pharmaceut Anal Acta 3:180. doi: 10.4172/2153-2435.1000180

Copyright: © 2012 Samir A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Five methods were developed for simultaneous determination of Salmeterol xinafoate (SAM) and Fluticasone propionate (FLU) without previous separation. In the first method (HPLC), a reversed-phase column and a mobile phase of acetonitrile: methanol (80:20 v/v) at 0.5 mLmin-1 flow rate was used to separate both drugs and UV detection at 220 nm. Linearity was obtained in concentration ranges of 50-500 μgmL-1 for Salmeterol xinafoate Fluticasone propionate. In the second method both drugs were determined using first derivative UV spectrophotometry, with zero crossing measurement at 352 and 269.5 nm for Salmeterol xinafoate and Fluticasone propionate, respectively. The third method depends on first derivative of the ratios spectra by measurements of the amplitudes at 334 and 337.5 nm for Salmeterol xinafoate and at 225 and 231.5 nm for Fluticasone propionate. Calibration graphs were established in the range of 4-28 μgmL-1 for both Salmeterol xinafoate and Fluticasone propionate. The fourth one depend on isosbestic point at 237.5 nm, while the content of Salmeterol xinafoate was determined by measuring the absolute value of the ultraviolet curves at 343 nm, without interference from Fluticasone propionate. All the proposed methods were extensively validated. They have the advantage of being economic and time saving. All the described methods can be readily utilized for the analysis of pharmaceutical formulations. The results obtained by adopting the proposed methods were statistically analyzed and compared with those obtained by official methods.

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