Reach Us +441474556909
Single Molecule Oscillations of an RNA/DNA Duplex in a Plasmonic Nanocavity | OMICS International | Abstract
ISSN: 2157-7439

Journal of Nanomedicine & Nanotechnology
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Single Molecule Oscillations of an RNA/DNA Duplex in a Plasmonic Nanocavity

Sagnik Basuray1, Avinash Pathak1, Biyan Chen1, Drew Menke2, Charles M Darr3, Keshab Gangopadhyay1,4, Peter V Cornish2* and Shubhra Gangopadhyay1*

1Department of Electrical and Computer Engineering, 349 Engineering Building West, University of Missouri, Columbia, MO 65211, USA

2Department of Biochemistry, 117 Schweitzer Hall, University of Missouri, Columbia, MO 65211, USA

3Department of Bioengineering, 349 Engineering Building West, University of Missouri, Columbia, MO 65211, USA

4Author Contributions Equality

*Corresponding Author:
Shubhra Gangopadhyay
141A Engineering Building West
University of Missouri
Columbia, MO 65211-2300, USA
Tel: 1-573-882- 4070
Fax: 1-573-884-0312
E-mail: [email protected]
Peter V Cornish
117 Schweitzer Hall
University of Missouri
Columbia, MO 65211, USA
Tel: 1-573-882-0443
Fax: 1-573-882-5635
E-mail: [email protected]

Received Date: April 06, 2015; Accepted Date: May 05, 2015; Published Date: May 15, 2015

Citation: Basuray S, Pathak A, Chen B, Menke D, Darr CM, et al. (2015) Single Molecule Oscillations of an RNA/DNA Duplex in a Plasmonic Nanocavity. J Nanomed Nanotechnol 6:293. doi:10.4172/2157-7439.1000293

Copyright: © 2015 Basuray S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


We report the visualization of single molecule dynamics in epifluorescence mode through extraordinary plasmonic enhancement provided by silver grating with embedded nanocavities. Cy3/Cy5-labeled DNA/RNA hybrid duplexes were affixed to SiO2-capped silver gratings produced by soft lithography process. Tracking single-molecule fluorescence revealed damped sub-1 Hz periodic Cy3 intensity fluctuations with strong dependence on the bulk MgCl2 concentration. Extreme concentration of electric field at the nanocavity edge induces plasmonic heating, which sets up convection deep within the nanocavity. Local fluctuations in Mg2+ ion concentration promote a bent or unbent duplex conformational state, respectively, by varying degrees of negative charge screening along the duplex backbone. These oscillations continue until the duplex conformational state stabilizes or the dyes bleach. This unique molecular behavior in the nanocavity could be used to study duplex complementarity, structural polymorphisms, and protein-nucleic acid interactions at the single molecule level.


Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri and Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry


[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version