Standardizing Harvesting Methods for Human Turbinate Mesenchymal Stem Cells from Surgical Tissue
- *Corresponding Author:
- Jihong Kim
MD, Kim Sung Soo ARCO ENT clinic
ARCO Sensorineural Laboratory
Anyang-Si, Kyunggi-Do, Korea
E-mail: [email protected]com
Received Date: March 28, 2017 Accepted Date: April 14, 2017 Published Date: April 17, 2017
Citation: Kim B, Kim M, Kim S, Kim M, Kim J (2017) Standardizing Harvesting Methods for Human Turbinate Mesenchymal Stem Cells from Surgical Tissue. Single Cell Biol 6: 160. doi:10.4172/2168-9431.1000160
Copyright: © 2017 Kim B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In accordance with many disease occurrences, stem cell research has become an important study. Mesenchymal stem cell (MSC) is one of representative materials of stem cell research. MSCs are multipotent progenitor cells that can differentiate into various cell types including osteocytes, adipocytes, chondrocytes, myocytes, stromal cells and neurons. MSCs have been derived from bone marrow, umbilical cord, umbilical cord blood, fat, and other body organs. Among them, human inferior turbinate and nasal septal cartilages are good materials for extraction of MSCs. For extraction of MSCs, we used MSC extraction protocol from ARCO sensorineural laboratory. In order to identified extracted MSCs, Immunofluorescence to CD90 and CD73 (or CD105) cell-surface markers of MSCs was performed using the MSC phenotyping kit (Miltenyi Biotec, Germany). hMSCs derived from inferior turbinate and nasal septal cartilage were shown positive results. Further study, more studies need to comparative analysis of differentiated target cells from our MSCs.