alexa Subclinical Diagnosis of Caseous Lymphadenitis Based on ELISA in Sheep from Brazil
ISSN: 2155-9597

Journal of Bacteriology & Parasitology
Open Access

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Research Article

Subclinical Diagnosis of Caseous Lymphadenitis Based on ELISA in Sheep from Brazil

Dayana Ribeiro1, Fernanda Alves Dorella2, Luis Gustavo Carvalho Pacheco3, Núbia Seyffert2, Thiago Luiz de Paula Castro2, Ricardo Wagner Dias Portela3, Roberto Meyer3, Anderson Miyoshi2, Maria Cecília Rui Luvizotto4 and Vasco Azevedo1,2*

1Laboratório de Genética Celular e Molecular, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

2Laboratório de Genética Celular e Molecular, Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

3Laboratório de Imunologia e Biologia Molecular, Departamento de Bio-Interação, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Brazil

4Laboratório de Patologia Veterinária, Departamento de Clínica, Cirurgia e Reprodução Animal, Universidade do Estado de São Paulo, Araçatuba, Brazil

*Corresponding Author:
Vasco Azevedo
Laboratório de Genética Celular e Molecular
Departamento de Biologia Geral
Instituto de Ciências Biológicas
Universidade Federal de Minas Gerais
Belo Horizonte, Brazil
Tel/Fax: 55-31-34092610
E-mail: [email protected]

Received date: June 10, 2013; Accepted date: July 05, 2013; Published date: July 11, 2013

Citation: Ribeiro D, Dorella FA, Pacheco LGC, Seyffert N, de Paula Castro TL, et al. (2013) Subclinical Diagnosis of Caseous Lymphadenitis Based on ELISA in Sheep from Brazil. J Bacteriol Parasitol 4:170. doi: 10.4172/2155-9597.1000170

Copyright: © 2013 Ribeiro D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Caseous lymphadenitis (CLA), caused by Corynebacterium pseudotuberculosis, is a chronic contagious disease that affects small ruminants and still remains an important problem for many lamb-producing countries. Animals are considered clinically infected when occurs abscesses in superficial lymph nodes. Visceral or internal form can coexist which no apparent clinical signs of infection are seen. The best procedure to avoid spread of the disease is elimination of infected animals. However, as the chronic and subclinical nature of the infection of CLA alternative methods are required for detection and screening. In this study, we described the performance of indirect Enzyme-Linked Immunosorbent Assay (ELISA) for diagnosis of CLA in asymptomatics sheep. Also, test culture and biochemical identification were achieved to confirm CLA infection. The serological diagnostic was performed in sheep symptomatics (n=50) and asymptomatics (n=374) from nine flocks. Analysis reported high positivity of 71% for ELISA in 85% of asymptomatic animal for CLA with a sensitivity of 88% and specificity of 31%. Results from ELISA test in asymptomatic animals against culture for caseous lymphadenitis were more specific (97%) and permitted to exclude healthy animals without symptoms. This study concluded that C. pseudotuberculosis infection could be widely disseminated in sheep flocks in Northwestern region of the state of São Paulo, Brazil and only one screening test is not enough. The association with indirect ELISA test and culture could better indicate the real problem of CLA in sheep flocks.

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