The Effect of Ozone Gas Using Different Remineralizing Materials on Non- Cavitated Caries-Like Lesions in Permanent Teeth
Objective: To evaluate in vitro, the effect of ozone gas followed by remineralizing materials on non-cavitated caries-like lesions in permanent teeth. Material and Methods: Sixty extracted sound premolars with standardized window on buccal and lingual surfaces were immersed in demineralizing solution. Each tooth was sectioned into two halves. The specimens were randomly divided into three equal groups (I, II, and III) (n=40), each group was subdivided into two subgroups (n=20), experimental [I(a), II(a), and III(a)] and control [I(b), II(b), and III(b)]. Subgroup I(a) was treated with ozone followed by fluoridated toothpaste, subgroup II(a) was treated with ozone followed by HealOzone remineralizing solution and oral hygiene patient kit, and subgroup III(a) was treated with ozone followed by fluoride varnish and fluoridated toothpaste. Their controls left untreated. After 4 weeks the specimens were evaluated using Scanning Electron Microscopy with Energy Dispersive X-ray Analysis (SEM-EDX) for identifying the elemental composition of the specimen. Results: There was significant increase in the mean Ca/P in the 3 test subgroups when comparing with their controls (PÃÂ0.05). The mean Zn values were significantly increased in subgroup II (a) (PÃÂ0.05) at confidence interval 95%. Conclusions: Ozone application followed by different remineralizing materials had the same effect on calcium uptake, although HealOzone remineralizing solution and patient kit had better effect on remineralization due to the presence of zinc.