alexa The Effect of Varying Culture Conditions on the Production of Antibiotics by Streptomyces spp., Isolated from the Amazonian Soil
ISSN: 2167-7972

Fermentation Technology
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

The Effect of Varying Culture Conditions on the Production of Antibiotics by Streptomyces spp., Isolated from the Amazonian Soil

Ingrid Reis da Silva1,2*, Mayra Kassawara Martins1, Clarice Maia Carvalho1, João Lúcio de Azevedo1,2 and Rudi Emerson de Lima Procópio1

1Microbiology Laboratory, Amazon Biotechnology Center - CBA, Manaus, Amazonas, Brazil

2Biotechnology, Federal University of Amazonas - UFAM, Manaus, Amazonas, Brazil.

*Corresponding Author:
Ingrid Reis da Silva
Microbiology Laboratory
Amazon Biotechnology Center
CBA and Biotechnology
Federal University of Amazonas - UFAM
Manaus, Amazonas, Brazil
Tel: +55 92 31824862
E-mail: [email protected]

Received date: July 19, 2012; Accepted date: July 26, 2012; Published date: July 28, 2012

Citation: da Silva IR, Martins MK, Carvalho CM, de Azevedo JL, de Lima Procópio RE (2012) The Effect of Varying Culture Conditions on the Production of Antibiotics by Streptomyces spp., Isolated from the Amazonian Soil. Ferment Technol 1:105. doi:10.4172/2167-7972.1000105

Copyright: © 2012 da Silva IR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



The genus Streptomyces is considered to be of great industrial importance because of its ability to produce secondary metabolites that account for 80% of the antibiotics currently in use. To optimise the production of antimicrobial compounds from three strains of Streptomyces spp. isolated from the Amazon’s soil, we investigated the influence of physical (temperature, pH, agitation and time) and chemical (concentrations of carbon and nitrogen) variables, according to a factorial statistical design consisting of three repetitions at the central point. During a period between five and twenty days of incubation, the temperature was varied between 20 and 40°C, the pH was varied between 4.5 and 8.5, and the agitation was varied between 100 and 300 rpm. The concentrations of carbon and nitrogen sources ranged from 5 to 15 g/L and 0.5 to 1.5 g/L, respectively, and the results were evaluated using the Response Surface Methodology (RSM). Our data showed that the most effective carbon sources were starch and glycerol and that the best sources of nitrogen were phenylalanine, ammonia sulphate, asparagine and peptone. The results of this study showed that the temperature, incubation time and the culture medium directly influenced the production of metabolites (antibiotics). These parameters can be modified for the optimisation and improvement of the fermentation process by increasing the production of the compound of interest. Each Streptomyces behaved differently, requiring specific conditions for the production of secondary metabolites.


Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version