The Immunogenicity of Peptoid-Protein Conjugates
Allison Case1,2#, Angela Desmond1,3#, Daniel Lopes1,4, Kelly Dye1,5, Kelly Mapes1,6, Stephen Ruback1, Iliodora Pop1,7, Jiyeun Kate Kim1,8, Pavitra Chakravarty1, Joan E. Smallshaw1, Laurentiu M. Pop1,9 and Ellen S.Vitetta1,10*
- *Corresponding Author:
- Ellen S. Vitetta
Cancer Immunobiology Center and Department of Immunology
University of Texas Southwestern Medical Center
Dallas, Texas, USA
E-mail: [email protected]
Received date: June 20, 2016; Accepted date: July 06, 2016; Published date: July 11, 2016
Citation: Case A, Desmond A, Lopes D, Dye K, Mapes K, et al. (2016) The Immunogenicity of Peptoid-Protein Conjugates. J Vaccines Vaccin 7:329. doi: 10.4172/2157-7560.1000329
Copyright: © 2016 Case A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
We demonstrate that a peptoid composed of five monomers and attached via a maleimide linker to a carrier protein elicits anti-peptoid, anti-linker and anti-carrier antibodies in rabbits. Specific anti-peptoid antibodies were affinity purified and used to reproducibly retrieve three specific peptoid-coupled beads from 20,000 irrelevant peptoid-beads using magnetic screening.