alexa The Organosulphur Composition of Allium Separated by Different Analytical Methods
ISSN: 2161-1009

Biochemistry & Analytical Biochemistry
Open Access

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The Organosulphur Composition of Allium Separated by Different Analytical Methods

Marius Pentea and Monica Butnariu*

Banat’s University of Agricultural Sciences and Veterinary Medicine, Romania

*Corresponding Author:
Butnariu M
Banat’s University of Agricultural Sciences and
Veterinary Medicine “Regele Mihai I al Romanei” Timisoara
Romania. 300645,Calea Aradului 119, Timis, Romania
E-mail: [email protected]

Received Date: September 22, 2014; Accepted Date: September 23, 2014; Published Date: September 30, 2014

Citation: Pentea M, Butnariu M (2014) The Organosulphur Composition of Allium Separated by Different Analytical Methods. Biochem Anal Biochem 3:e149. doi: 10.4172/2161-1009.1000e149

Copyright: 2014 Butnariu M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Various procedures have been examined for the separation of the compound formed in solution Alliacee cut plants. To prepare an extract, the product id squeezed through a nylon mesh, saturated in NaCl and shaken twice with dichloromethane, filtering and centrifuging each time. The dichloromethane extracts were combined, dried and concentrated in vacuum at room temperature and then analyzed by HPLC and FT–RMN and/or GC (modified injector). Supercritical carbon dioxide can also be used as solvent extraction. For the preparation of a distillate at room temperature, the homogenate is subjected to a distillation using an oil bath to keep the container at room temperature. The distillate is colored to –196°C, saturated with NaCl, extracted and analyzed. For a particular plant extracts and distillates obtained at room temperature, they were similar in terms of analytically.

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