The Synergy of Electrochemical Impedance Spectroscopy and Medicinal Technology
Edward P Randviir* and Craig E Banks
Faculty of Science and Engineering, School of Chemistry and the Environment, Division of Chemistry and Environmental Science, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, Lancs, UK
- *Corresponding Author:
- Edward P Randviir
Faculty of Science and Engineering
School of Chemistry and the Environment
Division of Chemistry and Environmental Science
Manchester Metropolitan University, Chester Street
Manchester M1 5GD, Lancs, UK
E-mail: [email protected] gmail.com, [email protected]
Received Date: November 27, 2014; Accepted Date: January 13, 2015; Published Date: January 16, 2015
Citation: Randviir EP, Banks CE (2015) The Synergy of Electrochemical Impedance Spectroscopy and Medicinal Technology. Pharm Anal Acta 6:336. doi: 10.4172/2153-2435.1000336
Copyright: © 2015 Randviir EP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A reversed phase high performance liquid chromatographic assay coupled with UV detection [239 nm] has been developed and validated for the determination of midazolam in rat plasma samples after transdermal administration. A liquid–liquid extraction was used to extract the compound from plasma sample. The separation was performed on a Hypersil ODS C18 column using a mobile phase composed of acetonitrile and 0.1% triethylamine aqueous solution [ 52:48, V/V], pumped at a flow rate 1.0 mL min-1. The calibration curves showed good linearity with correlation coefficient higher than 0.998 for all analytes in the range 0.10–10.0 μg mL-1. Accuracy in the measurement of quality control [QC] samples was in the range 95–107% of the nominal values. The intra-day and inter-day precisions in the measurement of QC samples were less than 10% coefficient of variation. The developed method is suitable for quality control of midazolam in their mixtures and in transdermal delivery system pharmaceutical preparations. The validated assay was found to be suitable for the pharmacokinetic study of midazolam in rats with transdermal administration.