alexa Tissue Responses Exhibited by Biomphalaria Alexandrina Snails from Different Egyptian Localities Following Exposure to Schistosoma Mansoni Miracidia | OMICS International | Abstract
ISSN: 2155-9597

Journal of Bacteriology & Parasitology
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Research Article

Tissue Responses Exhibited by Biomphalaria Alexandrina Snails from Different Egyptian Localities Following Exposure to Schistosoma Mansoni Miracidia

A.H. Mohamed1, A.T. Sharaf El-Din2*, A.M. Mohamed2 and M.R. Habib2

1Zoology Department, Faculty of Science, Menoufiya University.

2Medical Malacology Lab., Theodor Bilharz Research Institute. P.O. Box 30, Imbaba Egypt

*Corresponding Author:
Dr. A.T. Sharaf El-Din
Department of Environmental Researches and Medical Malacology
Theodor Bilharz Research Institute. P.O. Box 30, Imbaba Egypt
Tel: +2 0107981467
Fax: +2 35408125
E-mail: [email protected]

Received date: September 06, 2010; Accepted date: October 28, 2010; Published date: October 30, 2010

Citation: Mohamed AH, El-Din ATS, Mohamed AM, Habib MR (2010) Tissue Responses Exhibited by Biomphalaria Alexandrina Snails from Different Egyptian Localities Following Exposure to Schistosoma Mansoni Miracidia. J Bacteriol Parasitol 2:104. doi: 10.4172/2155-9597.1000104

Copyright: © 2010 Mohamed AH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Streptococcus pneumoniae is an important cause of bacterial keratitis, an infectious disease of the cornea. This study aimed to determine the importance of pneumolysin (PLY), a pneumococcal virulence factor, in keratitis using a clinical keratitis isolate (K1263) and its isogenic mutant deficient in PLY (K1263?PLY) and determine the effect of these strains on primary rabbit corneal epithelial (RCE) cells. Each strain was injected into the corneal stromas of rabbits, clinical examinations were performed, and the recovered bacterial loads were determined. Bacterial extracts were exposed to RCE cells, and morphology and viability were assessed. The mutant strain deficient in PLY, K1263?PLY, caused significantly lower ocular disease scores than the parent strain (K1263), although a higher bacterial load was recovered from corneas infected with the mutant strain. Histological examination showed increased inflammatory cells in the anterior chamber and increased edema in eyes infected with the parent strain. RCE cells exposed to the parent strain had significantly decreased cell viability and showed increased evidence of cellular damage. This study confirms that in a strain that can cause clinical keratitis, PLY is a significant cause of the damage associated with pneumococcal keratitis. It also shows for the first time that the results from an in vitro model using RCE cells correlates with in vivo results thereby establishing a less invasive way to study the mechanisms of pneumococcal keratitis.

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