Validated HPLC Method for the Determination of JWU1497 in Rat Plasma and Its Application to a Comparative Pharmacokinetic Study of the Free Base and Hydrophosphate Salt Forms of JWU1497
Tae Kon Kim* and Oui Hyun Jung
College of Science & Engineering, Jungwon University, 85 Munmu-ro, Geosan-gun, Chungbuk, South Korea
- *Corresponding Author:
- Tae Kon Kim
College of Science & Engineering
Jungwon University, 85 Munmu-ro
Geosan-gun, Chungbuk 367-700, South Korea
E-mail: [email protected]
Received Date: July 12, 2015 Accepted Date: September 02, 2015 Published Date: September 09, 2015
Citation: Kim TK, Jung OH (2015) Validated HPLC Method for the Determination of JWU1497 in Rat Plasma and Its Application to a Comparative Pharmacokinetic Study of the Free Base and Hydrophosphate Salt Forms of JWU1497. J Bioequiv Availab 07:267-269. doi:10.4172/jbb.1000251
Copyright: © 2015 Kim TK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In this study, a sensitive and reliable method for the quantitation of JWU1497 in rat plasma was developed and validated using high performance liquid chromatography (HPLC). The pharmacokinetics of 2 forms of JWU1497, namely the base form and the hydrophosphate salt form, were investigated in rats. The 2 forms were orally administered to rats and the plasma concentrations of JWU1497 were determined using HPLC. The JWU1497 base and hydrophosphate salt forms showed similar pharmacokinetic profiles in terms of their maximum plasma concentration (Cmax) and area under the concentration-time curve (AUC). The time to peak concentration (Tmax) of the base form was slightly greater than that of the salt form, but this difference was not statistically significant. These results suggest that the JWU1497 base and hydrophosphate forms are pharmacokinetically equivalent in rats, and thus the base form could be used in various JWU1497 formulations as a substitute for the existing JWU1497 hydrophosphate form.