Voltammetric Determination of Isoniazid using Cyclic Renewable Mercury Film Silver Based ElectrodeMarek Szlósarczyk1*, Robert Piech2, Beata Paczosa Bator2, Anna Maslanka1, Wlodzimierz Opoka1 and Jan Krzek1
- *Corresponding Author:
- Marek Szlósarczyk
Department of Inorganic and Analytical Chemistry
Jagiellonian University, Medical College, 30–688 Kraków
Medyczna 9, Poland
Tel: +48 12 6205480
Fax: +48 12 6205480
Received date: August 17, 2012; Accepted date: November 23, 2012; Published date: November 26, 2012
Citation: Szlósarczyk M, Piech R, Bator BP, Maslanka A, Opoka W, et al. (2012) Voltammetric Determination of Isoniazid using Cyclic Renewable Mercury Film Silver Based Electrode. Pharmaceut Anal Acta 3:189. doi: 10.4172/2153-2435.1000189
Copyright: © 2012 Szlósarczyk M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A new cathodic voltammetry method for the determination of isoniazid based on the cyclic renewable mercury film silver based electrode (Hg (Ag) FE) is presented. The effects of various factors such as: preconcentration potential and time, pulse height, step potential and supporting electrolyte composition are optimized. The calibration graph is linear from 5 nM up to 500 nM (68.55 μgL-1). For Hg (Ag) FE with a surface area of 9.7 mm2 without time consuming preconcentration the limits of detection LOD and quantification LOQ were 4.1 nM and 10.5 nM of isoniazid, respectively. The repeatability of the method at a concentration level of the analyte as low as 0.5 nM, expressed as RSD is 3.6% (n=6). The proposed method was successfully applied in analysis of isonizid in simple and composed pharmaceutical formulations.