alexa Analysis Of Glutathione And Other Low Molecular Weight Antioxidants Using Colorimetric Detection On Smartphones
ISSN: 2157-7110

Journal of Food Processing & Technology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

3rd European Food Safety & Standards Conference
October 24-25, 2016 Valencia, Spain

Irena Vobornikova and Miroslav Pohanka
University of Defense, Czech Republic
Posters & Accepted Abstracts: J Food Process Technol
DOI: 10.4172/2157-7110.C1.054
Abstract
Glutathione is a tri-peptide composed of cysteine, glutamic acid and glycine. Its level is discussed in wine and must be in connection with loss of aroma and browning. This low molecular weight antioxidant plays important role as a scavenger of free radicals and reactive oxygen compounds. Smartphones are commonly used devices that can serve as colorimetric detectors. We designed and made on three-dimensional printer as well holder for smartphone glutathione analysis. In this paper, we present smartphone as an analytical tool for the determination of glutathione. Other low-molecular weight antioxidant´s influence was studied. On the paper target, low-molecular weight antioxidants were added to the samples of glutathione and finally 5, 5´dithiobis-(2-nitrobenzoic acid) (DTNB) reagent to form light yellow, yellow or orange drop. Red, green, blue (RGB) channel intensity data were evaluated in silico. The result was compared with standard Ellman spectrophotometric method. The picture analysis based on the camera in smartphone gave us promising results. The red and green channel intensity data were almost the same for all the range of concentration for glutathione. Blue channel intensity data decreased from the concentration 0 mmol/L to 16mmol/L of glutathione. The limit of detection (S/N=3) was 0.4 mmol/L for glutathione while 250 μmol/L ascorbic acid or 150 μmol/L Trolox had minimal influence on glutathione analysis. We can say that smartphone based method seems to be suitable for the glutathione analysis. The smartphones are widely spread in the population; the machine is portable that is why this assay can be carried out in the field or at home. There is low cost for the instrument equipment, the own manipulation is easy and fast. Other advantage of this method is robustness and reliability.
Biography

Irena Vobornikova is working as a Researcher at the University of Defense, Czech Republic. Her experience includes various programs, contributions and participation in different countries for diverse fields of study. Her research interests reflect in her wide range of publications in various national and international journals.

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords