Journal of Microbial & Biochemical Technology

Antimicrobial activity of clove oil against microbes isolated from water

International Conference and Expo on Water Microbiology & Novel Technologies

July 18-19, 2016 Chicago, USA

Prati Pal Singh and Daljit Kaur

National Institute of Pharmaceutical Education and Research, India

Posters & Accepted Abstracts: J Microb Biochem Technol

Abstract:

We studied the antimicrobial activity of clove oil against drug-resistant bacteria in water collected from nearby regions. The bacteria were identified and assessed of antimicrobial resistance by using BD Pheonix 100 automated microbiology system. Klebsiella pneumoniae, Enterobacter cloacae, Klebsiella oxytoca, Acinetobacter lwoffii/haemolyticus, Escherichia coli, Arcanobacterium haemolyticum, Bacillus cereus, Pediococcus pentosaceus, Gardnerella vaginalis, Macrococcus caseolyticus, Dermacoccus nishinomiyaensis, Staphylococcus cohnii, Staphylococcus gallinarum, Bacillus megaterium, Streptococcus anginosus and Streptococcus porcinus were isolated. Almost all the Gram-negative isolates were resistant to cefazolin, cephalexin, cefoxitin, cefotaxime, ampicillin, amoxicillinclavulanate, trimethoprim-sulfamethoxazole, nalidixic acid and tetracyclin and nitrofurantoin. On the other hand, the Grampositive isolates showed tolerance to cefoxitin, ampicillin, penicillin G, oxacillin, amoxicillin-clavulanate, teicoplanin, quinupristindalfopristin, vancomycin, clindamycin, erythromycin, ciprofloxacin, rifampin and tetracyclin. The Gram-negative bacteria showed highest resistant to ampicillin (76%), cefazolin (64%), cephalexin (52%) and nalidixic acid (40%), respectively. Similarly, up to 80% Gram-positive isolates were resistant to clindamycin and 60% to cefoxitin, ampicillin, penicillin G and erythromycin. The antibacterial activity of clove oil was tested in 5% aqueous DMSO and worked well in the order 1:1>1:5>1:10>1:20. The multiple drug-resistant Enterobacter cloacae isolate was relatively more sensitive (inhibition zone diameter (IZD): 19 mm) to clove oil than Escherichia coli (IZD 18 mm). For Staphylococcus sciuri, Klebsiella pneumonia and Staphylococcus gallinarum the IZDs were 14.5 mm, 13.5 mm and 11 mm, respectively. Clove oil appeared to have some significant antimicrobial activity which can be used for therapeutic purposes.

Biography :

Email: drppsingh2005@gmail.com