alexa
Reach Us +1-217-403-9671
Bioanalytical Method Development Of The Metabolite 3-desmethylthiocolchicine In Human Plasma By LC-MS/MS | 8361
ISSN: 0975-0851

Journal of Bioequivalence & Bioavailability
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Bioanalytical method development of the metabolite 3-desmethylthiocolchicine in human plasma by LC-MS/MS

International Conference & Exhibition Bioequivalence and Bioavailability

Dr. Sangita Agarwal

ScientificTracks Abstracts: Bioavailability & Bioequivalence

DOI: 10.4172/0975-0851.1000001

Abstract
T hiocolchicoside (TCS) is a muscle relaxant agent with anti- inflammatory and analgesic actions and is absorbed rapidly from the gastrointestinal tract, after oral administration and peak plasma concentrations are observed within approximately 1 h. Analytical methods published for the determination of TCS to date, are either non specific radioimmunoassay techniques or use enzymatic hydrolysis of the TCS to 3-desmethylthiocolchicine (3DMT). TCS is metabolized so rapidly after oral administration that it becomes impractical to determine its concentration in plasma over the periods generally used for bioequivalence studies A sensitive, reliable and reproducible method has been developed for the determination of 3-desmethylthiocolchicine (3DMT) in plasma, using high performance liquid chromatographic separation with tandem mass spectrometric detection (LC-MS/MS).. The plasma samples were extracted with ethyl acetate and separated on a Phenomenex Gemini column with a mobile phase consisting of methanol: water containing 0.5% formic acid (95.5:4.5 v/v) at a flow rate of 0.45 ml/min. Detection was achieved by an Applied Biosystem, API 2000 mass spectrometer (LC-MS-MS) set at unit resolution in the multiple reaction monitoring mode. Turbo Ion Spray ionization was used for ion production. The MRM transition of m/z 402.10 ? 360.10 and m/z 354.00 ? 214.00 were used to measure 3DMT and Aceclofenac (IS) respectively. The method was validated over the concentration range of 2 to 50ng/ml for 3DMT in human plasma. The mean recovery for 3DMT was 79%, with a lower limit of quantification set at 1ng/ml. The increased selectivity of mass spectrometric (MS-MS) detection allowed us to distinguish between TCS and its major metabolite 3DMT in human plasma, thereby giving more insight about the pharmacokinetics of the drug in human
Biography
Top