alexa Coagulation Tests Affected By Acute Phase Reactants Such As CRP And Factor VIII | 13544
ISSN: 2155-9864

Journal of Blood Disorders & Transfusion
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

International Conference on Hematology & Blood Disorders

Jun Teruya
Accepted Abstracts: J Blood Disord Transfus
DOI: 10.4172/2155-9864.S1.004
Abstract
Glycoprotein VI (GPVI), a key receptor for platelet activation by collagen, plays a critical role in hemostasis in vivo . The GPVI- collagen interaction has been clearly involved in the formation of thrombi on atherosclerotic lesion that are responsible for acute ischemic events. The neutralization of the collagen- GPVI interaction thus represents a promising strategy to develop novel antithrombotic agents. Two approaches were evaluated in this study:1) Blocking GPVI with antibody fragments, and 2) Triggering a binding competition using a novel synthetic peptidomimetic. 1. Starting from a well-characterized hybridoma secreting an IgGk, a recombinant single chain antibody fragment (scFv) was designed and characterized. It retains the binding proprieties of the parental IgG (e.g. high affinity, neutralization of GPVI- collagen). The purified scFv inhibits the platelet aggregation induced by collagen in conditions which mimic the arterial flow. Humanized scFvs were finally obtained and can be used as building units to create larger antibody fragment with therapeutic potential. Furthermore, the strategy developed here is simple, efficient and straightforward and could also be used for humanizing other antibodies. 2. Using a molecular evolution procedure, a set of peptidomimetics of human GPVI were identified and synthesized. One of them binds to collagen with a nanomolar affinity in vitro and competes with GPVI for binding to collagen. Collagen accumulation (fibrosis) was detected in vivo using a radiolabelled peptide and this capacity can be used to develop a non- invasive isotopic imagery method for the diagnostic and the evolution of fibrosis.
Biography
Julien Muzard completed his Ph.D. in Biotechnology (University Paris Diderot) in 2007 and spent five years as postdoc. The first was at Paris- Tech, followed by positions at the Center for Molecular Innovation and g Discovery, University College Dublin. He is now a fellow in Computational Biology at CNAM University, Paris. His interests are mostly related to in silico , in vitro and in vivo aspects of binding recognition and the creation of molecular entities from scratch. He is coauthor on about 20 publications and main inventor on 5 patents granted in various disciplines (basic research, diagnostic and therapeutics).
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version