alexa Co-expression Of PgNHX1 And AtAVP1 Improves Tolerance To Both Water And Salt Stress In Transgenic Rice
ISSN: 2168-9881

Agrotechnology
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

2nd International Conference on Agricultural & Horticultural Sciences
Radisson Blu Plaza Hotel, Hyderabad, India February 03-05, 2014

Sushma M Awaji, Prashantkumar, S Hanjagi, Rohini Sreevatsa and V R Sashidhar
Posters: Agrotechnol
DOI: 10.4172/2168-9881.S1.008
Abstract
Abiotic stresses such as drought and salinity are serious threats to modern agriculture. Climate change will likely make many places worse in terms of water availability and soil salinization which will have adverse impacts on food production in agriculture. Rice is the most preferred staple food for human population all over the world and it is frequently affected by several abiotic stresses including drought, salinity and cold. A number of transgenic improvements for abiotic stress tolerance have been achieved in rice. The present study reports that, the over expression of PgNHX1-AtAVP1 in rice improves drought and salt tolerance. When T 2 seeds were grown on media containing different concentrations of PEG (8000), -12 and -17 bars, the PgNHX1-AVP1 expressing rice plants showed enhanced root growth, which was hindered in the wild type seedlings. To screen putative T 2 plants for salt tolerance, stringent salt screening test was followed and root and shoot growth of T 2 putative transformants was used as a selection criterion. Some of the transgenics showed significantly higher root and shoot growth compared to wild type when they were subjected to 350 mM NaCl stress. To confirm the integration of the transgene in putative T 2 transgenic plants, PCR and RT-PCR analysis were performed. The results showed that all the selected seedlings were PCR positives and four lines were positive for RT-PCR analysis. Physiological studies viz., chlorophyll estimation, membrane stability index, cell viability test and K+/Na+ ratio were also conducted to assess their levels of stress tolerance in the T 2 generation. Some of the T 2 transformants showed lower percent reduction in chlorophyll content, higher membrane stability, cell viability and maintained higher K+/Na+ ratio after PEG and NaCl treatment compared to wild type. The results clearly demonstrated that transgenic rice plants over expressing PgNHX1-AVP1 had superior stress tolerance capacity as compared to non-transformed plants.
Biography

Sushma M. Awaji has completed her post graduation at the age of 23 years from University of Agricultural Sciences, GKVK, Bangalore. At present she is pursuing Ph.D. degree with INSPIRE fellowship in the Department of Crop Physiology, UAS, GKVK, Bangalore, under the supervision of Dr. V. R. Sashidhar, with the thesis problem entitled A gene pyramiding approach to improve salt tolerance in Rice involving tonoplast and plasma membrane transporters. She has published 3 papers in international journals and presented 4 posters in international conferences.

image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

g[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords