alexa Computational Mining Of Transcriptomes For Transcription Factors Controlling Various Biological Processes In Podophyllum Spp
ISSN: 2168-9881

Agrotechnology
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

2nd International Conference on Agricultural & Horticultural Sciences
Radisson Blu Plaza Hotel, Hyderabad, India February 03-05, 2014

Pawan Kumar, Tarun Pal, Varun Jaiswal, Hemant Sood and Rajinder S Chauhan
Posters: Agrotechnol
DOI: 10.4172/2168-9881.S1.008
Abstract
Sinopodophyllum hexandrum Royle ( syn. P. emodi Wall) is a critical endangered medicinal plant native to alpine and sub- alpine regions of Himalayan countries like India, Nepal, Pakistan, China and Bhutan (2500-4200m) commonly known as bankakri or Giriparpat in North- Western Himalayan region. This medicinal plant is well known for anticancer compounds podophyllotoxin and its derivatives. Till date, Podophyllum species have been the source of podophyllotoxin, the preliminary compound for the production of the semi-synthetic anti-cancer drugs etoposide (VP-16-213), teniposide (VM-26), etopophos, Pod-Ben- 25, Condofil, Verrusol, Warticon etc. which are used in the treatment of leukaemia, lung, testicular cancer and rheumatoid arthritis. The biology of various physiological and biochemical processes contributing to total plant biomass and yield of podophyllotoxin is not fully understood, thereby, limiting genetic intervention approaches. In addition to biosynthetic pathways, the transcription factors (TFs) and transcription regulators (TRs) play important role in regulating the production of podophyllotoxin. Podophyllum hexandrum and Podophyllum peltatum are the two important species of Podophyllum having 4.3% and 0.25% podophyllotoxin respectively. Different tissues (leaf, stem, root, rhizome) of both the species synthesizes different amounts of podophyllotoxin and its derivatives. Podophyllotoxin was found in the leaf tissue of P. p e l t a t u m but not in P. hexandrum . Current study is aimed at identification of TFs/TRs through the computational mining of transcriptomes from different tissues of P. hexandrum and P. p e l t a t u m . Transcriptome data of P. hexandrum (2, 27, 885 contigs) and P. p e l t a t u m (1, 47, 960 contigs) were collected from the Medicinal Plant Transcriptomics Database. TFs/TRs were identified according to presence of InterPro domains using PlantTFcat. All contings were annotated to proteins (according to possible open reading frames) and searched through InterProScan. PlantTFcat classified these contings into 108 known TF and TR families. We identified total 3652 and 4412 TF/TR-encoding transcripts (contigs) which belonged to 97 and 95 TF/TR families in P. hexandrum and P. p e l t a t u m respectively. Differentially expressed TFs/TRs in leaf, stem, root and rhizome tissues of P. hexandrum and P. peltatum were identified according to contribution of tissue specific small reads in TFs/TRs encoding contigs. 3477, 3617 and 3517 TFs/TRs encoding transcripts were found in the leaf, stem and rhizome tissues of P. hexandrum respectively. Similarly 3383, 3307 and 3824 TFs/TRs encoding transcripts were found in leaf, stem and root tissue P. p e l t a t u m respectively. These transcripts are expected to be involved in various tissue specific biological processes like photosynthesis, disease resistance, secondary metabolite synthesis etc. 175, 81 and 35 TF/TR encoding contigs were absent in leaf, stem and rhizome tissues of P. hexandrum . Similarly 1029, 1105 and 588 TF/TR encoding contigs were absent in leaf, stem and root tissues of P. p e l t a t u m . The differentially expressed TFs/TRs may provide valuable information related to tissue specific biochemical processes occurring in leaf, stem, root and rhizome e.g. podophyllotoxin synthesis is known to be rhizome/root specific and TFs/TRs uniquely present in rhizome can be further explored for their role in podophyllotoxin biosynthesis. This study will provide a platform for better understanding the biology of physiological and biochemical processes in Podophyllum species ( P. hexandrum and P. p e l t a t u m ).
Biography

Pawan Kumar has worked as a Research Fellow in the CSIR-Institute of Himalayan Bioresource Technology from April 2011 to January 2013. Currently, he is pursuing PhD in the Dept. of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Himachal Pradesh, India. His current research interests emphasizes on the Metabolomics, Metabolic Engineering, Plant Tissue Culture, Computational Biology, Plant Molecular Biology.

image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

immun[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords