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nfectious disease, commonly caused by bacterial pathogens, is now
the world?s leading cause of premature death and third overall
cause behind cardiovascular disease and cancer. Urinary Tract
Infection (UTI), caused by pathogenic E.coli bacteria Escherichia (e.g.
O157:H7) infection can rapidly produce a powerful toxin, resulting in
hemorrhagic colitis. Other pathogenic bacteria (Cholera, Salmonella,
Pseudomonas etc.) also produce harmful toxins for the human system.
A gold-based nano-biosensor (or, GNWA-antibody-bound gold Nano
Wire Arrays) has been developed for quick quantitative determination
of the number of bacteria present in UTI by using a detection method
by Electrical Impedance Studies (EIS). Preliminary results indicate that
the GNWA biosensors (attached to C11 -linkers) can detect as few
as 50 E.coli cells on a sensor area of 0.2 cm2 . GNWA C11-linkers are
attached to a specific antibody raised against the specific bacterial
antigens. Attachment of a specific linker with a specific chain length is
important for optimum binding of the bacterial cells. Our present study
is optimized for E.coli only. This quick quantification and identification
of the bacteria for binding with its specific antibodies (under specific
optimal binding condition) would provide quick treatment of the
patients with proper antibiotics or other bacteria?killing drugs
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