Clinical Microbiology: Open Access

Detection of periodonto pathogens in patients with chronic periodontitis: Culture versus real-time PCR

4^th International Conference on Clinical Microbiology and Microbial Genomics

October 05-07, 2015 Philadelphia, USA

Fredy Gamboa, Luis Garcia, Mayra Martinez, Alveiro Erira, Dabeiba-Adriana Garcia and Adriana Acosta

Pontifical Xavierian University, Colombia

Posters-Accepted Abstracts: Clin Microbiol

Abstract:

Introduction: Periodontitis is a chronic infectious disease associated with a complex microbiota. Porphyromonas gingivalis, Aggregatibacter actinomycetem comitas, Prevotella intermedia and other sub-gingival species have been related to periodontal disease. Aim: The objective of this study was to investigate the presence of P. gingivalis, P. intermedia and A. actinomycetes comitans in patients with chronic periodontitis by the method of anaerobic culture compared to the real-time PCR technique. Methods: Bacterial isolation by anaerobic culture and real-time PCR for P. gingivalis, P. intermedia and A. actinomycetem comitans was performed in 90 chronic periodontitis patients who attended clinics at the Pontificia Universidad Javeriana. Bacterial sampling was done with paper points in the gingival sulcus, transport took place in thioglycolate broth and primary culturing was carried out on Wilkins-Chalgren agar in anaerobic conditions. For real-time PCR technique, DNA extraction from clinical specimen was performed by the method DNAzol�?�?�?® and the real-time PCR was carried out according to a standardized protocol. Results: By culturing P. gingivalis, P. intermedia and A. actinomycetem comitans were respectively present in 44(48.9%), 30(33.3%) and (4.4%) of the 90 samples. In contrast method by real-time PCR, P. gingivalis, P. intermedia and A. actinomycetem comitans were detected respectively in 78(86.7%), 52(57.8%) and 18(20%) of the 90 samples. Conclusion: The three periodonto pathogens were most frequently detected by the method of real-time PCR; y 2. In some cases both techniques are complementary in detecting the periodonto pathogens.

Biography :

Fredy Gamboa finished his Doctorate in 1997 at the Autonomous University of Barcelona-Spain. He is the coordinator of the Oral Microbiology branch at Dental Research Center, Javeriana University in Bogota-Colombia. Currently, he is working in the microbiological and molecular characterization of important bacteria in dental caries, periodontal and endodontic disease. In oral microbial ecology he is characterizing the micro-biome present in preschool children before and after a process of education. Also he has interest in the isolation and characterization of microorganisms of the oral cavity for use as probiotics and antagonistic action.

Email: gamboa@javeriana.edu.co