Journal of Bacteriology & Parasitology

Effect of ciprofloxacin and levofloxacin alone or in combination with N-acetylcysteine on biofilmproducing Enterococcus faecalis: Contaminated root canals of extracted human teeth

3^rd International Congress on Bacteriology and Infectious Diseases

August 04-06, 2015 Valencia, Spain

Rashid Ramazanzadeh, Mohammad Rastegar Khosravi and Masoumeh Khonsha

Scientific Tracks Abstracts: J Bacteriol Parasitol

Abstract:

Introduction: Biofilm producing-Enterococcus faecalis remains a major challenge in the disinfection of an infected root canal
system. The aim of this in vitro study was to investigate the antibacterial effect of ciprofloxacin (CIP), levofloxacin (LEV) and
their combination with N-acetylcysteine (NAC) in root canals infected with E. faecalis biofilms.
Methods: Ninety one single root teeth were prepared. The antimicrobial effectiveness of seven-day intra canal medication
with increasing concentrations (MIC to 1000MIC) of calcium hydroxide (CaOH2), CIP, LEV, CIP plus NAC (8 mg/ml) and
LEV plus NAC was evaluated using the roots infected with E. faecalis for 30 days. Colony-forming unit (CFU) counting was
performed. Biofilm formation and structural changes were monitored using scanning electron microscopy (SEM).
Results: All the chemotherapeutic agents were significantly more effective than CaOH2 (P<0.05). There was no significant
difference between combined treatment with NAC and single antibiotic treatments (P>0.05) except for LEV plus NAC which
showed significantly higher reduction in CFU/ml than LEV at concentrations of 50 MIC and above (P<0.05). The effectiveness
of the antibiotics was significantly positively correlated with their concentrations (r>0.8; P=0.000). SEM examination
confirmed the formation of mature biofilms and a complete accordance between reductions in CFU/ml and destruction of
biofilm structures was found.
Conclusions: The local application of CIP and LEV and in vitro synergism of LEV and NAC led to more antimicrobial efficacy
than CaOH2 against E. faecalis biofilms in root canals of extracted human teeth.

Biography :

Rashid Ramazanzadeh currently Faculty of Medicine at Cellular & Molecular Research Center and Microbiology Department, Kurdistan University of Medical
Science, Iran.