alexa Efficacy Of Phage Typing As A Diagnostic Bio-marker Tool In MRSA Outbreaks And Staphagetherapy Evaluation
ISSN: 2327-5073

Clinical Microbiology: Open Access
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

3rd International Conference on Clinical Microbiology & Microbial Genomics
September 24-26, 2014 Valencia Convention Centre, Spain

Nagwa Mohamed Amin Aref and Eman Kamel Al-Digs
Accepted Abstracts: Clin Microbial
DOI: 10.4172/2327-5073.S1.016
Abstract
173 Staphylococcus aureus isolates were collected from microbiology lab hospitals in Riyadh and Jeddah. Distribution of methicillin resistant Staphylococcus aureus (MRSA) isolates among clinical departments and isolations from different specimens showed that the wound infections resemble the highest percentage of the clinical sites (26%). The all over percentage of MRSA topical infections (70%) was higher than MSSA infections.All isolates were sensitive to vancomycin which is the drug of choice for MRSA infections. Mupirocin showed low degree of resistance, which was 6%. Susceptibility to other commonly used antibiotics was variable. Resistance oxacillin was the highest 34%. Some degrees of resistance to sulphomethoxazole/ trimethoprim and fusidic acid (34%) were noticed. Terms like boarder line resistant, low-level mutation, and boarder- line susceptible had also been obtained in our studied isolates of S. aureus for methecillin using Minimum Inhibitory Concentration (MIC) test; which was observed in the percentage of MRSA isolates in our data that had MICs of 256μg/m1 (30.83%). Studying the distribution of phage type patterns among Staphylococcus patients isolates in Saudi cohort; it was found that only 30.56% were typable with different degree, and 69.7% were non typable. Differentiation increased in the phage typing, and genome typing yielding 20 and 23 different S. aureus types, respectively. A 61.71% of the MRSA isolates were genetically typed by PFGE resulting one type of MRSA with one genetic difference. Genome typing by PFGE was a powerful tool not only for strain identification but also for the resolution of clonal relationships of S. aureus strains. Observation on UV induction of S. aureus strains showed that the number of infective centers increased by more than 100%. As seen in our electron micrographs, our studied phages designated from ph1 to ph10; five of these phages ph2, ph4, ph5, ph7, and ph10 were morphotype A, present in Myoviredae group, their DNAs were in the range 43.6 kb-48.5 kb that is very close to this size range with minor differences in phage strain. Ph9 and ph10; had more than one band in their pattern. A mixture of ten phages differed in their virulence to MRSA isolates were used with all patient isolates (30) that were lysed completely by spot-test. Phage therapy for 30 patients of MRSA in different skin infection cases showed that phage therapy was highly effective. Using a mixture of 10 phages daily (7-10 days), treatment gave us a close success rate of 90%.
Biography
Nagwa Mohamed Amin Aref has completed her PhD in Ain Shams University, Cairo, Egypt as a channel system study with University of Hohenheim, Institute fur phytomidizine, Stuttgart, Germany. In 1984, in Virology and Virus diagnoses and postdoctoral studies from Michigan State University, Department of Botany and Plant Pathology and Center of Electron Optics in East Lansing, USA, participating in the detection of Plant viral disease of the research program 1984-1989. Coinvestigated at AGERI (Agriculture Genetic Engineering Research Institute), Ministry of Agriculture, Egypt, 1990-1996 in UNDP project entitled: ?Engineering tomato transgenic plants resistant? in contribution with Scripps Research Institute, San Diego, USA. She was an Exchange Visitor Professor for a research scholarship in Electron Imaging Center, California NanoSystem Institute (CNSI, University of California (UCLA) in Los Angeles, USA. She has published more than 40 publications in reputed journals supervising postgraduate students and serving as an editorial board member of reputed journals. She is working now as a Professor in Botany and Microbiology Department in College of Science, King Saud University.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version