alexa Establishment Of Transient Transformation Protocol Along With Comparison Of Regeneration Potential Following Introduction Of Antiporter Genes In Peanut (Arachis Hypogaea L.)
ISSN: 2157-7471

Journal of Plant Pathology & Microbiology
Open Access

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2nd International Conference on Plant Science & Physiology
June 26-27, 2017 Bangkok, Thailand

Kashmery Khan and Aparna Islam
BRAC University, Bangladesh
Posters & Accepted Abstracts: J Plant Pathol Microbiol
DOI: 10.4172/2157-7471-C1-006
Abstract
Background: Peanut is a cash crop having outstanding food values. Therefore, it can be used as a supplementary food for hunger and malnutrition affecting Asian countries like Bangladesh. But peanut yield is substantially reduced for abiotic stresses like salinity. As a result, salt tolerant peanut has to be improved to ensure better production where the success of conventional breeding severely hampered due to its cleistogamous nature of pollination. Thus, Agrobacterium-mediated genetic transformation may lead the solution. Aim: Present study was examined with three Bangladeshi peanut varieties, such as, BINA Chinabadam 2, BINA Chinabadam 4 and BINA Chinabadam 6 where two explants, named, decapitated half embryo and embryonic leaflet were transformed with vectors, such as, pBI121, pK7WG2_OsNHX1_1.6 & pK7WG2_AtNHX1_1.6. Results & Conclusion: Decapitated half embryo showed better in vitro regeneration potential than leaflet explant. Hence, transformation study was conducted with 1 day old decapitated half embryo where highest percentage of GUS positive explants were found when OD600 was more than 1 at 60 minutes of infection and 3 days of co-cultivation duration in transient assay. Regeneration efficiency on selection media containing 50 mg/l kanamycin was higher in almost all three varieties. In contrast to this, untransformed control explants were able to resist upto 20 mg/l of kanamycin. Moreover, average number of putative transformed shoots was gradually dropped with the increased concentration of kanamycin. Shoots which survived upto 150 mg/l kanamycin were transferred into rooting media for root induction. Finally transformation efficiency was evaluated by calculating the percentage of GUS positive plant parts, such as, leaf, stem and roots among the putatively transformed plantlets. In the current experiment, effect of salinity on peanut seed germination was also observed. The germination rate steadily decreased with the increase of NaCl in the media and finally no germination was observed in MS media supplemented with 150 mM of NaCl and above.
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