Journal of Pharmacovigilance

Evaluation of hepatoprotective activity of Ixora coccinea against xenobiotics induced liver damage in alloxan induced diabetic rats

2^nd International Conference and Exhibition on Pharmacovigilance & Clinical Trials

November 18-19, 2013 Hilton San Antonio Airport, TX, USA

Ramesh Alluri, Rajashekar Perusomula and Kashivishwanath Routhu

Accepted Abstracts: J Pharmacovigilance

Abstract:

The objective was to evaluate hepatoprotective activity of Ixora coccinea (IC) against xenobiotics induced liver damage in alloxan induced diabetic rats. Wistar rats of either sex (250?5 gm) were divided into 5 groups. Animals of (Group-1) were non diabetic and received 4 ml/kg p.o allyl alcohol. Diabetes was induced by alloxan (160 mg/kg i.p). Diabetic animals were divided into control (Group-2), ally alcohol (Group-3), IC (600 mg/kg p.o + ally alcohol 4 ml/kg p.o Group-4). Allyl alcohol administration in diabetic animals showed increased necrosis index compared to non diabetic group. Treatment of animals with IC 600 mg/kg showed dose dependent reduction of necrosis index. In separate group of non diabetic and diabetic animals thioacetamide (300 mg/kg i.p) was administered to induce hepatotoxicity on day 6. Group-1 non diabetic control (distill water p.o), Group-2 non diabetic thioacetamide, group 3 diabetic control, Group-4 diabetic thioacetamide control (300 mg/kg i.p), Group-5 (thioacetamide+IC 600 mg/kg p.o). Blood samples and liver samples were collected on day 8. Thioacetamide produced increase in serum AST & ALT levels in both non diabetic and diabetic animals. There was significant increase in serum AST & ALT levels observed in diabetic animals when compared to non diabetic animals. IC (600 mg/kg, for 8 days) showed significant reduction in serum AST & ALT levels compared to thioacetamide (300 mg/kg) group. Histopathology results correlated with biochemical parameters. It is concluded that IC (600 mg/kg) showed hepatoprotective effect in both allyl alcohol and thioacetamide induced hepatotoxicity in both diabetic and non diabetic animals.