Journal of Vaccines & Vaccination

Genetic association of interferon gamma induced protein-10 (IP-10), CXCL-10 gene polymorphisms with TB pleurisy susceptibility in South Indian population

7^th Middle East - Global Summit and Expo on Vaccines & Vaccination

September 28-29, 2015 Dubai, UAE

Sheikh Ghousunnissa

Lepra Blue Peter Public Health & Research Centre, India

Posters-Accepted Abstracts: J Vaccines Vaccin

Abstract:

Background: Chemokines are involved in both innate and adaptive responses. Tuberculosis pleurisy may be caused due to spread of infected macrophages/ monocytes trafficking from the lung. CXCL-10 known as Interferon gamma-induced protein 10 (IP-10) or small-inducible cytokine 10 is 8.7 Kda protein, which is secreted in response to IFN-�?³ by monocytes, endothelial cells and fibroblasts. It has chemo-attraction for monocytes/macrophages T cells, NK cells and dendritic cells in promotion of T cell adhesion to endothelial cells. Aim: In the present study, we investigated whether polymorphisms in CXCL-10 gene have any role contributing to disease manifestation of TB pleurisy. Materials & Method: Two SNPs in CXCL-10 promoter (â�?�?1447A>G and â�?�?135G>A) were genotyped in 186 TB Pleurisy, 159 Pulmonary TB patients and 205 healthy subjects by PCR-RFLP. Disease associations were examined by Ï�?2 Fisher exact test. Results: Polymorphisms in the promoter region (â�?�?135G>A) genotype GA and allele G frequency were significantly high (p<0.05) in TB subjects compared to healthy controls. The genotype AA and allele A was significantly low (p<0.05) with TB pleurisy subjects compared to healthy controls. The frequency of haplotype A-G combination of (1447A>G and â�?�?135 G>A) was significantly high (p<0.05) in TB pleurisy. Conclusions: Our results reveal that genotype GA and allele G frequency at (â�?�?135G>A) position were strongly associated with rendering susceptibilityto tuberculous pleurisy. The GA genotype may be a useful genetic marker for early detection of the disease in high risk individuals.

Biography :

Sheikh Ghousunnissa is pursuing her PhD final year under the Supervision of Dr. Vijaya Lakshmi Valluri from Genetics Department, Osmania University.

Email: nissa782006@gmail.com