Pharmaceutica Analytica Acta

Green synthesis of silver nanoparticles, characterization and study its wound healing property

5^th International Conference and Exhibition on Pharmaceutics & Novel Drug Delivery Systems

March 16-18, 2015 Crowne Plaza, Dubai, UAE

Mehta Tejal, Shah Jigna, Shram Shrama Om Prakash, Vashi Ronak and Patel Smit

Posters & Accepted Abstracts: Pharm Anal Acta

Abstract:

Silver nanoparticles is one of the paramount model in the gamut of nanotechnology materials due to its insurmountable properties like antimicrobial agents, catalysts etc. which provoked intensive research interest of researchers to use it in various applications. Current research is focused to augment the wound healing and antioxidant activity of aqueous extract of Tridax procumbens in combination of broad anti-bacterial activity of silver nanoparticles. Green synthesis of silver nanoparticles has proven to be better alternate over chemical methods which render product with bio compatible attributes whilst giving a cost effective and environmental friendly process. In the present research, silver nanoparticles were prepared using aqueous extract of Tridax procumbens having polysaccharides constituents which causes stepwise reduction of silver ions to synthesize silver nanoparticles. Nanoparticles were characterized using ultra violet spectrophotometry (surface plasmon resonance) and dynamic light scattering (DLS) methods. Average nanoparticle size was found between 90-120nm and particle size distribution was found narrow. For ease in use of above mixture of nanoparticles and plant extract for wound healing activity, topical gel was prepared using carbopol as polymer. Sprague dolly rat was used as excisional wound model. Rats were divided in four groups, controlled, standard (silver sulfadiazine), plant extract and silver nanogel, having 6 rats in each group. Percentage wound contraction, epithelization time and time taken to remove scar tissue was taken as evaluation parameters and their results were statistically analyzed. It was found that rats treated with silver nanogel shown better and faster wound healing compared to other groups which proves increased wound healing property of Tridax procumbens in combination with silver nanoparticles.