alexa High Level Secretory Expression Of Brazzein In Yeast | 18304
ISSN: 2157-7110

Journal of Food Processing & Technology
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

3rd International Conference and Exhibition on Food Processing & Technology

Jinkyung Lim, Mimi Kim and Kwang-Hoon Kong
Posters: J Food Process Technol
DOI: 10.4172/2157-7110.S1.006
Abstract
Brazzein, a sweet-taste protein, is a small protein derived from the African plant Pentadiplandra brazzeana Baillon. In previous study, we constructed the expression system for recombinant brazzein in yeast Kluyveromyces lactis which is recognized as GRAS. Brazzein expressed in yeast expression system shows high yield and can be simply purified due to yeast?s secretion pathway. In addition, as folding procedure of proteins takes place in endoplasmic reticulum(ER) in eukaryotes, proteins expressed in yeast are automatically folded properly. However, there exist four disulfide bonds in the structure of brazzein which may lead to the misfolding of the protein in high level. Thus, by over-expressing protein disulfide isomerase which catalyzes disulfide bond formation and isomerization of protein in the ER, we induced proper protein folding so that the secretion of recombinant brazzein can be increased. As a result, the expression level of the recombinant brazzein increased approximately 1.5 times more than that of previous expression system and the amount of misfolded/unfold recombinant brazzein remaining inside the cell decreased. For much higher expression of the recombinant brazzein, we also replaced LAC4- PB I promoter existing in commercial pKLAC2 vector with wild-type LAC4 promoter. As LAC4-PB I promoter which lacks function in bacteria shows the reduced level of transcription in yeast, we restored the original promoter, LAC4, in order to increase the level of transcription of brazzein gene. The yield of the recombinant brazzein expressed in final expression system was approximately 1.7 times more than in previous expression system. We expect that diverse brazzein variants would be applied to this expression system for commercial production due to its high yield.
Biography
Jinkyung Lim earned a Bachelor?s degree from Chung-Ang University in Korea. She is currently pursuing her Master course in Biochemistry. She has published a paper regarding GST enzyme in journal and has researched yeast expression system of weet-taste protein brazzein.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7