Journal of Microbial & Biochemical Technology

In silico analysis of gene encoding catechol 2,3-dioxygenase (XylE) in Pseudomonas sp. degrading crude oil bacteria

World Congress and Expo on Applied Microbiology

August 18-20, 2015 Frankfurt, Germany

Hadiatullah

Brawijaya University Malang, Indonesia

Posters-Accepted Abstracts: J Microb Biochem Technol

Abstract:

xylE gene is the gene encoding Catechol 2,3-dioxygenase which responsible to degrading of aromatic compounds. The objective of this paper to observe the relationship of the Pseudomonas sp. based on amino acid sequence with the potency of degraging crude oil. The method used were collecting amino acid sequence of xylE gen of Pseudomonas sp. which obtained from GeneBank (www.ncbi.nm.nih.gov), analysis of polimorfism obtained by using BioEdit after allignment, The phylogenetic trees were constructed based on protein sequences of xylE gene MEGA5.05 software, The conserved domains of amino acid relationship from 10 kinds of Pseudomonas were identified by using BioEdit software. The phylogenetic tree showed that Pseudomonas putida is similar to Pseudomonas stutzeri and has a close similarity with Pseudomonas pseudoalcaligenes based on amino acid relationship. The data indicated that Pseudomonas putida shares genetic similarities with Pseudomonas stutzeri, and the both of them probably belong to the same potency of degarading crude oil. Based on the polimorfism site, Pseudomonas mendocina and Pseudomonas veronii have less potency than other Pseudomonas species in degarding crude oil.