alexa Investigation Of The Cellular Uptake Of Functionalised Carbon Nanoparticles
ISSN: 2157-7439

Journal of Nanomedicine & Nanotechnology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

13th International Conference and Exhibition on Nanomedicine and Pharmaceutical Nanotechnology
July 24-25, 2017 | Rome, Italy

Badriah Hifni, Eugene Dempsey, Jeremy C Simpson and Susan Quinn
University College Dublin, Ireland
Posters & Accepted Abstracts: J Nanomed Nanotechnol
DOI: 10.4172/2157-7439-C1-050
Abstract
Nanomaterials possess excellent structural, electronic and optical properties and are seen to offer huge potential in nanomedicine applications. Due to their biocompatible chemical composition, carbon nanoparticles have received significant attention in recent years, yet many fundamental questions still remain about their mechanism of internalisation by cells. The aim of this project is to address this lack of knowledge for two forms of carbon particles, Carbon Nanohorns (CNHs) with high surface area and chemical inertness, and carbon nanodots (C-dots) that display good water dispersibility, chemical inertness and high biocompatibility. A range of chemical synthesis methods were used to generate C-dots. These were characterised using a range of techniques including AFM, DLS and FTIR and their cellular uptake was assessed by utilising in vitro HeLa cell-based assays to determine how internalisation occurs and the pathways they follow in cells. Three families of C-dots have been successfully synthesised by three different approaches, namely microwave, electrochemical and solvothermal synthesis. The resulting C-dots showed emission profiles in the blue, green and red parts of the spectrum under different excitation conditions. A maximum emission peak at 500 nm is observed when the green C-dots are excited at 440 nm, whereas the CNHs emit at 568 nm when excited at 551 nm. Upon characterisation, AFM and DLS methods determined the average diameter of the C-dots and CNHs to be ~ 4 nm and 198 nm respectively. MTT cytotoxicity assays suggest that the blue and red C-dots do not induce any significant toxicity to cells, while the green C-dots at 25 μg/mL cause an approximately 60% reduction in cell viability. Overall, different classes of carbon nanoparticles with low cytotoxicity can be readily synthesised and subsequently utilised to study their trafficking in mammanlian cells. Future work will focus on unravelling the specific pathways involved in this trafficking process with a view to better designing carbon nanomaterials to act as vehicles for drug delivery.
Biography

Badriah Hifni received her Master’s degree at King Abdulaziz University of Jeddah (Saudi Arabia) for her work on the genotoxicity and cytotoxicity of carboplatin in somatic and germ cells of adult male swiss albino mice under the supervision of Dr. Salwa Mohammad. Currently, she is in her third year of Doctor of Philosophy (PhD) program at University College Dublin. The focus of her PhD is the investigation of the cellular uptake of functionalized carbon nanoparticles under the supervision of Prof. Jeremy Simpson and Dr. Susan Quinn.

Email: [email protected]

image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords