alexa Isolation And Comparative Genomics Of Soil Bacteria With Antibacterial Activity Against Methicillin-resistant Staphylococcus Aureus | 15153
ISSN: 2155-9597

Journal of Bacteriology & Parasitology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

International Congress on Bacteriology & Infectious Diseases

Shamima Nasrin, Erin Schmale and Mark R. Liles
Posters: J Bacteriol Parasitol
DOI: 10.4172/2155-9597.S1.003
Abstract
The emergence of multidrug-resistant pathogens has increased the demand for discovery of novel antibiotics. Soil microbial communities are a great resource for natural products buta majority of them have not been explored yet due to the bacterial unculturability. We cultured a collection of over 500 bacterial and fungal isolates from soil usinglow-strength (1/200th) nutrient agar supplemented with soil extract with extended period of incubation. Bacterial diversity analysis using 16S rRNA gene sequences of newly cultured isolates revealed that they represent diverse bacterial genera affiliated with the phyla Acidobacteria, Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. Two isolates, designatedas A115 and F4, were found to inhibit the growth of pathogenic methicillin-resistant Staphylococcus aureus (MRSA). The isolate A115, member of the genus Streptomyces, produces pink pigments after incubation for more than ten days. The isolate F4, identified as Nonomuraea, produces a high molecular weight (>100kDa), heat stable reddish pigment with anti-MRSA activity.Whole genome sequencing and bioinformatics prediction demonstrated that A115 and F4 encode a total of 32 and 28 secondary metabolite biosynthesis gene clusters, respectively. A115 encodes Type I, II, and III PKS pathways, whereas F4 encodes Type I and III PKS pathways. The biochemical structure of the active anti-MRSA compounds are currently being characterized usingliquid chromatography?mass spectrometry (LC/MS). This study identified novel bacterial isolates with anti-MRSA activity and demonstrates the utility of novel cultivation techniques in obtaining previously uncultured and phylogenetically diverse soil microorganisms, some of which express potent bioactive secondary metabolites.
Biography
Shamima Nasrin, is a Ph.D. student in Microbiology at Auburn University. She received her Master?s degree in Biology from Tuskegee University in 2010. She participates in many academic research projects involved with both prokaryotes and eukaryotes.She was awarded Cancer Research fellowship award in 2009 and Cellular and Molecular biosciences fellowship in 2010. She has published several scientific articles and book chapters, and has presented research papers at several scientific meetings. Her current research focus is to discovery of antibacterial compounds against Methicillin-resistant Staphylococcus aureus (MRSA) from soil microorganisms using culture-dependent and culture?independent approaches.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version