Clinical Microbiology: Open Access

Klebsiella pneumoniae carbapenemase producing-KPC: Antibiotic activity of a secondary metabolite produced by Pseudomonas aeruginosa

3^rd International Conference on Clinical Microbiology & Microbial Genomics

September 24-26, 2014 Valencia Convention Centre, Spain

Galdino Andrade

Accepted Abstracts: Clin Microbial

Abstract:

The multidrug resistant organisms are the major challenge of medicine and resulting in an important loss for human health around the world. The limitations therapeutic of patients with Klebsiella pneumoniae carbapenemase KPC producing infection is a great problem that result in a high morbidity and mortality rates. It is clear the urgent need of new and more efficacious compounds for specific treatment of infections caused by K. pneumoniae KPC-producing. The aim of this study evaluated the antibiotic activity of F3d fraction obtained from secondary metabolite produced by Pseudomonas aeruginosa LV strain against clinical isolates of K. pneumoniae KPC-producing. The results showed that minimum inhibitory concentration of F3d (62.5 μg mL-1) killed the cells on viability test after 30 minutes of incubation. It was observed with the cell viability test using LIVE/DEAD BacLight kit after 8 hours that the proportion of cell died is bigger than the remaining in the culture treated with minimum inhibitory concentration dosis, also, no cell growth was seen on Muller Hinton agar after 14 hours of incubation in the presence of this fraction. The minimum inhibitory concentration dosis of F3d fraction also showed antibiotic activity against biofilm of K. pneumoniae -KPC producing strain after 24 hours incubation. The treatment with F3d fraction made pronounced morphological alterations of the K. pneumoniae -KPC planktonic and biofilm cells, showing that this compound has a great potential to develop a new antibiotic to use on the control of infections caused by this pathogenic bacteria and also acting in a biofilm formation.

Biography :

Galdino Andrade has completed his PhD from Universidad de Granada and Postdoctoral studies from University of Surrey, School Biological Science and from USDA at Oregon, USA. He is the head leader of Microbiology Department, Microbial Ecology Lab. He has published more than 35 papers in reputed journals and has been serving as an editorial board member of repute.