Clinical Microbiology: Open Access

Molecular epidemiology of Staphylococcus aureus with ERIC-PCR method

3^rd International Conference on Clinical Microbiology & Microbial Genomics

September 24-26, 2014 Valencia Convention Centre, Spain

Rashid Ramazanzadeh, ShadiehAbdollahi and ServehZamani

Accepted Abstracts: Clin Microbial

Abstract:

Background and Objectives: S.aureus is one of the most significant etiological agents responsible for healthcare-associated infections. The aim of this study was to show the genetic relationship in S.aureus isolates and their transmission pattern between hospitals. Materials and Methods: 90 S.aureus strains, isolated from hospitalized patients in the intensive care unit and infectious wards of Besat and Toohid hospitals, Sanandaj. Antimicrobial susceptibilities were determined by the disc diffusion method, Methicillin resistance was done by agar screen test and the resistance inducible by the D-Test. By ERIC-PCR technique relationship of strains was determined based on the similarities between DNA fingerprints by using Jaccards coefficient in the SAHN program of the NTSYS-pc software. Results: Fourteen different antimicrobial patterns were observed. 46.7% of the strains were susceptible to all antimicrobials tested. The ERIC?PCR profiles allowed typing of the 90 isolates into 75 ERIC-types which were grouped into eleven main clusters (C1?C11). The Fourth group with the largest number was formed 17 strains. Agreement between antibiotic patterns and rep-profiles was not observed for most isolates. Conclusion: The results of our study also showedthat most of Staphylococcusisolated produced different genomic fingerprintpatterns, therefore , dissemination source of infection is different. Keywords: S.aureus, ERIC?PCR, resistance