Pharmaceutica Analytica Acta

Nanoencapsulation of Annona vepretorum essential oil displays enhanced antitumor activity

5^th International Conference and Exhibition on Pharmaceutics & Novel Drug Delivery Systems

March 16-18, 2015 Crowne Plaza, Dubai, UAE

Daniel P Bezerra1, Larissa M Bomfim1, Leociley R A Menezes2, Ana Carolina B C Rodrigues1, Rosane B Dias1, Clarissa A Gurgel Rocha1,3, Milena B P Soares1,4, Albertino F S Neto3, Magaly P Nascimento5, Adriana F Campos5, Lidercia C R C e Silva3 and Emmanoel V Costa2

Posters & Accepted Abstracts: Pharm Anal Acta

Abstract:

Annona vepretorum Mart. (Annonaceae), popularly known as ?bruteira?, has both nutritional and medicinal uses. The aim of this study was to investigate the chemical composition and antitumor potential of the leaf essential oil of A. vepretorum alone and complexed with β-cyclodextrin in a nanoencapsulation. The essential oil was obtained by hydrodistillation using a Clevenger-type apparatus and analyzed by GC-MS and GC-FID. In vitro cytotoxicity of the essential oil and some of its major constituents (spathulenol, ocimene, α-phellandrene, α-pinene and o-cymene) for tumor cell lines from different histotypes was evaluated using the alamar blue assay. Furthermore, the essential oil in vivo effectiveness was demonstrated in mice inoculated with B16-F10 mouse melanoma. The essential oil presented bicyclogermacrene, spathulenol, ocimene, α-phellandrene, α-pinene and o-cymene, as major constituents. The essential oil and spathulenol presented promising cytotoxicity. Importantly, the nanoencapsulation of the essential oil led to a higher tumor growth inhibition. In conclusion, the leaf essential oil of A. vepretorum presents bicyclogermacrene, spathulenol, ocimene, α-phellandrene, α-pinene and o-cymene, as major constituents and has both in vitro and in vivo anticancer potential, which was enhanced by nanoencapsulation.