Journal of Microbial & Biochemical Technology

Preliminary effect of curcumin loaded liquid crystal precursor mucoadhesive system against Candida albicans with and without association of photodynamic therapy

Joint Event on 4^th World Congress and Expo on Applied Microbiology & 2^nd International Conference on Food Microbiology

November 29-December 01, 2017 Madrid, Spain

Jessica Bernegossi, Livia Nordi Dovigo, Jeffersson Trigo Gutierrez, Camila Cristina De Foggi, Ewerton Garcia de Oliveira Mima, Carlos Eduardo Vergani and Marlus Chorilli

Sao Paulo State University (UNESP), Brazil

Posters & Accepted Abstracts: J Microb Biochem Technol

Abstract:

Oral candidiasis is caused by yeast infection Candida spp. in soft tissues of the oral cavity, resulting mainly from imbalance in the oral microbiota, changes in pH and salivary flow. This study attempted to overcome this problem by developing, characterizing, and evaluating the in vitro antifungal action of a CUR-loaded liquid crystal precursor mucoadhesive system (LCPM) for buccal administration. For the evaluation of LCPM, biofilms were prepared with C. albicans (ATCC 90028) in a 96-well flat bottom plate. Biofilms were treated with LCPM and with CUR in DMSO 10%. After 20 minutes, pre-irradiation time, plate 2 was irradiated with LED (dose 10.07 J/cm2). All wells were washed 2 times with PBS and sterile saline was added, which was collected by sterile mini swabs for the complete withdrawal of biofilm after treatment. Thereafter, the swabs were transferred to microtubes containing 900 μl of saline, and stirred for cell detachment, with further serial dilutions (10-1 to 106). Aliquots of the dilutions were plated on petri dishes containing SDA. After 24 hours of incubation at 37° C, the colonies grown on the plates were counted and the number of CFU/mL was calculated. The results exhibited that curcumin-loaded in LCPM showed no inhibition, but when dissolved in 10% DMSO and associated with photodynamic therapy reduced 1.05 log10 compared to control of untreated biofilm. Possibly the formulation had low release of curcumin, which may have caused the unexpected result.

Biography :

Jessica Bernegossi has completed MSc at School of Pharmaceutical Sciences from Sao Paulo State University – UNESP (Araraquara- Brazil). Currently she is a PhD student in the same university having her research financed by Sao Paulo Research Foundation (FAPESP). She has published nine papers in reputed journals and has been serving as a reviewer for Journal of Oral Microbiology.