Journal of Microbial & Biochemical Technology

Prevalence of Anal and Oral HPV infection in the screening high-risk population of HIV positive MSM in the Czech Republic

Joint Event on 4^th World Congress and Expo on Applied Microbiology & 2^nd International Conference on Food Microbiology

November 29-December 01, 2017 Madrid, Spain

Jana Nemcova, Katerina Cerna, Jana Smahelova , Jana Hercogova, Filip Rob and Ondrej Ondic

Biopticka laborator, Czech Republic

Posters & Accepted Abstracts: J Microb Biochem Technol

Abstract:

An infection with high-risk human papillomavirus (HR-HPV), particularly HPV16, is essential for inducing most carcinomas in anal canal and oropharyngeal area. HIV infected individuals are at increased risk for developing HPV driven cancers. Sexual practices play an important role in the acquisition of oral and anal HPV infections. Men who have sex with men (MSM) feature with the highest prevalence of anal and oral HPV. This study publishes first data about Czech HIV positive MSM patients attending the anal cancer screening program.Liquid-based cytological samples (LBC) and oral rinse and gargles of 40 HIV positive MSM were analyzed with Anyplex II HPV28 detection kit and PCR targeting E1 gene of a broad spectrum of HPV types. Additionally, Aptima HPV Assay was performed from LBC.Oral HPV infection was detected in 32,5 % and HPV16 in 5 % of the subjects. Anal HPV infection was found in 82,5 % of the subjects; 70 % of patients were infected with a high-risk HPV type and aproximately half of them presented positivity with Aptima indicating trancriptionally active HR-HPV infection. HPV16 was found in 17,5 % of individuals. Type-specific concordance between oral and anal mucousa was very low.Our data indicate that anal and oral HPV prevalence in Czech HIV-positive MSM is high, similarly to western studies of this high-risk population. Anal HR-HPV infection accompanying the suspect anal cytology will result in the further examination of the patient via colposcopy and biopsy. Currently, there is no specific management of patients with oral HRHPV infection.

Biography :

Jana Nemcova has completed her PhD at the age of 31 years from Charles University in Prague, Faculty of Medicine in Pilsen. She is the deputy of the head of department of molecular genetics in Biopticka laboratory and junior scientist in Biomedical Center of the Faculty of Medicine in Pilsen. She is an author or co-author of 39 articles published in peer-reviewed international scientific magazins.