Clinical Microbiology: Open Access

Sequencing and phylogenetic analysis based on hypervariable (loop 1) region of hexon gene of Pakistani isolates of avian adenoviruses from IBH-HPS cases in 2015

3^rd Global Microbiologists Annual Meeting

August 15-17, 2016 Portland, Oregon, USA

Basit Jabbar, Rehman Shahzad, Nadia Naseer, Iqra Jabbar, Faisal Amin and Bakht Sultan

University of the Punjab, Pakistan
Grand Parent Lab Lahore, Pakistan

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

Molecular techniques coupled with bioinformatics analysis have been shown to be a rapid, cost-effective and efficient strategy for diagnosis and serotype characterization of fowl adenoviruses. The study involved diagnosis and characterization of fowl adenoviruses from the inclusion body hepatitis-hydropericardium syndrome cases in Pakistan in 2015. Fowl adenovirus DNA was isolated and hexongene region was amplified by PCR and amplicons were sequenced by Sanger dideoxy sequencing. A total of 8 adenovirus hexon gene loop 1 region sequences were used in subsequent phylogenetic analysis with the previous isolates identified from BLAST. Serotype 11 was diagnosed in 87% and serotype 1 in 10% of the cases while the least identified serotypes were serotype 8 (4%) and serotype 4 (2%). Sequences were submitted to NCBI. Query sequences together with the respective homologues were retrieved in FASTA format and imported to MEGA 7.0 for multiple sequence alignment using Clustal-W tool. Neighbor joining method was used to infer evolutionary history and 1000 bootstrap replicates were applied. Tajima-Nei method was selected to compute evolutionary distances, shown as number of base substitutions per site. Exclusion of alignment gaps resulted in 487 positions in the final dataset and 32 sequences were used to build the phylogenetic tree. Results of bioinformatics analysis indicated close relationship of the query sequences with the previous isolates of fowl adenoviruses from Pakistan, India, China, Austria, Italy, Russia, US and Canada.

Biography :

Basit Jabbar has completed MS in Biotechnology from Institute of Biochemistry and Biotechnology, University of the Punjab in 2014 and is working in University of the Punjab as a Research Associate and Visiting Lecturer. He has worked on cloning of thermostable bacterial α-amylase during his BS degree from Institute of Industrial Biotechnology from Government College University Lahore and on Human Genetics during his MS degree.

Email: basit618@hotmail.com