Brain Disorders & Therapy

Specific platelet microRNA signature for Dementia with Lewy bodies as promising biomarker

5^th International Conference on Brain Disorders and Therapeutics

November 29-30, 2017 | Madrid, Spain

Ana Gamez Valero

Universitat Autònoma de Barcelona, Spain

Scientific Tracks Abstracts: Brain Disord Ther

Abstract:

Dementia with Lewy bodies (DLB) shows overlapping features with Alzheimer disease (AD) leading to its misdiagnosis and hindering its adequate treatment. It is well established that microRNAs play an important role in neurodegeneration and they can be found in brain and the central nervous system. Since 30 years, the presence of RNA (mRNA and microRNA) in platelets has attracted attention and different mRNA expression patterns have been reported to be altered in human diseases. Our main objective was to identify disease specific mRNA and miRNA biosignatures through the analysis of platelet rich plasma (PRP) obtained from DLB patients (n=31), AD patients (n=10) and age-matched control individuals (n=31). After isolation of mRNA and miRNA from PRP, we analyzed SNCA and GBA mRNA expression levels in DLB patients and controls by reverse-transcription real time PCR; next generation sequencing was applied for miRNA discovery in the same samples. 22 miRNAs were differentially expressed and selected to be validated by real time qPCR. From those, 9 were considered as potential biomarkers for DLB being evaluated in a group of AD patients and independent groups of control and DLB individuals. Interestingly, miRNAs levels seemed to correlate with clinical symptoms and to correlate inversely with SNCAmRNA expression in DLB patients. These results, although replication and follow-up with larger, carefully characterized cohorts are needed, represent an integrated miRNA and mRNA profile in platelet rich plasma that is likely to provide noninvasive biomarkers for the differential diagnosis of DLB versus AD.

Biography :

Ana Gámez-Valero obtained her degree in Biotechnology in the University Pablo de Olavide (Seville). During the four years, he had the opportunity to study a year in France where she started research experience in the Histology Department of the medical school (Nîmes). In 2013, he obtained an MSc in Advanced Genetics from the Universidad Autónoma of Barcelona. Since 2015, she is carrying out PhD project named “Identification of specific biosignatures from plasma samples for differential diagnosis of dementia with Lewy bodies” in the IGTP Institute in Badalona, having already published four research articles.