Modern Chemistry & Applications

Study of the best Trichoderma reesei support for hydrolysis of cellulose

Joint Event on 19^th International Conference on Medicinal Chemistry & Multi Targeted Drug Delivery & International Conference on Catalysis and Pyrolysis

November 05-06, 2018 | San Francisco, USA

Kurizara Sayamnikorn, Tidarat Komolvanich, Apanee Luengnaruemitchai, Thanyalak Chaisuwan and Sujitra Wongkasemjit

Chulalongkorn University, Thailand

Posters & Accepted Abstracts: Mod Chem Appl

Abstract:

Bioethanol, one of the outstanding biofuels, is not only a renewable bio-based resource but also interesting to research for improving better properties of the enzyme needed in the hydrolysis process. The purpose of this work is to study how to reuse the Trichoderma reesei enzyme used in the hydrolysis step for bioethanol production by immobilization technique using various silica supports, namely, SBA-15, TUD-1 and MCM-48. The amount of the adsorbed enzymes was determined by UVvisible spectrophotometry. Among those studied supports, SBA-15 impressively provided 100% enzymatic adsorption owing to its larger pore diameter of 6.14 nm, which is large enough to accommodate enzyme molecules inside the pore channel. Various parameters, viz. temperature, pH, time and amount of the support for optimizing the immobilized enzyme were investigated. Both supports and the immobilized T. reesei on SBA-15 support were characterized by N2 adsorption-desorption. The amount of monomeric sugar after the hydrolysis process was analyzed by high-performance liquid chromatography (HPLC) to determine the optimal conditions for the hydrolysis process using the immobilized T. resssi on the SBA-15 support.

Biography :

Kurizara Sayamnikorn was born and raised in Bangkok, Thailand. Obtaining her Bachelor degree from Silpakorn University and Master degree in Polymer Science from the Petroleum and Petrochemical College, Chulalongkorn University. Her thesis research was focused on immobilizing enzyme on various supports for the hydrolysis application. She enjoys fashion, basketball and traveling.

E-mail: dsujitra@chula.ac.th