Journal of Neonatal Biology

The role of miR-182 in regulating pineal clock expression after hypoxiaâ??ischemia brain injury in neonatal rats

20^th International Conference on Neonatology and Perinatology

December 04-06, 2017 | Madrid, Spain

Xin Ding

Children′s Hospital of Soochow University, China

Scientific Tracks Abstracts: J Neonatal Biol

Abstract:

Circadian rhythm disorder is a common neurological deficit caused by neonatal hypoxic-ischemic brain damage (HIBD). However, little is known about its underlying mechanisms. Our previous studies revealed a significant elevation of clock genes at the protein, but not mRNA, levels in the pineal gland after neonatal HIBD. To investigate the mechanisms of posttranscriptional regulation on clock genes, we screened changes of miRNA levels in the pineal gland after neonatal HIBD using high-throughput arrays. Within the miRNAs whose expression was significantly down-regulated, we identified one miRNA (miR182) that targeted the 3�??-untranslated region (3�??-UTR) of clock, a key component of clock genes, and played a crucial role in regulating clock expression after oxygen�??glucose deprivation in primarily cultured pinealocytes. Our findings therefore provide new insight on studies of therapeutic targets for circadian rhythm disturbance after neonatal HIBD.

Biography :

Xin Ding, MD, PhD, is a visiting scholar at Boston Children’s Hospital of Harvard University. She works under the guidence of Xing Feng, the Secretary-General and permanent member of committee of Pediatrics Branch of Chinese Medical Association. She has published nearly 10 papers in reputed journals on circadian rhythm disturbance.