Journal of Bacteriology & Parasitology

Toxoplasmosis and abortion: Pro and anti-inflammatory cytokines gene expression of the host immune cells

2^nd International Conference on Parasitology

August 01-03, 2016 Manchester, UK

Amany A Abd El-Aal, Walid Sayed El-Sherbiny, Samar Sayed Attia, Mohamed Zaki Badr, Mona Said El-Sherbeni, Asmaa A Abd El-Aal and Inas Z Abdel Aziz

Cairo University, Egypt
Ain Shams University, Egypt

Scientific Tracks Abstracts: J Bacteriol Parasitol

Abstract:

This work aimed to study gene expression profile of pro and anti-inflammatory cytokines in Toxoplasma sero-positive women presenting with repeated abortion compared to Toxoplasma sero-positive multiparous women with no history of abortions. The study groups included; group I: 19 cases of repeated abortions with no successful pregnancy, group II: 28 multiparous women, without any abortions and group III: 14 multigravida with successful pregnancies & suffered few abortions. All samples were positive for anti Toxoplasma IgG and IgM. Reverse transcription quantitative PCR (RT-qPCR) system was optimized to measure gene expression of pro-inflammatory IFN-γ & TNF-α and anti-inflammatory IL-10 & TGF-β cytokines. All studied cytokines were significantly up-regulated in group I, with remarkable bias towards IFN-γ, denoting a predominant inflammatory state in group I, compared to the other groups. In group II; the 4 tested cytokines were approximately in a balanced state with a little shift towards the antiinflammatory cytokines (IL-10 & TGF-β). Furthermore, a significant positive correlation was detected between pro-inflammatory together (p=0.002, r=0.96) and between anti-inflammatory cytokines together (p=0.0008, r=0.99) in group II, indicating the harmonized and synchronized expression of both pro & anti-inflammatory cytokines. In group III, IL-10 was relatively higher versus other cytokines with negative correlation with IFN-γ (p=0.006, r=0.93), indicating the possible regulatory role of IL10 in saving some of pregnancies�?? outcomes in group III. The results of the present study suggests a potential role of these cytokines in the upcoming prognostic or/and therapeutic concerns in women suffering from repeated abortion associated with chronic toxoplasmosis.

Biography :

Amany A Abd El Aal has completed her MD degree in Medical Parasitology from Faculty of Medicine, Cairo University in collaboration with NAMRU-3 (Navy American Military Research Unit-3), Abbasia, Cairo, from 1990 to 1995. She is a Professor since 2006 and currently working in Medical Parasitology Department as well as Army Forces of College of Medicine (AFCM). She is a Member in the scientific committee responsible for promotion of professor & assistant professor working in the field of Medical Parasitology in Egyptian universities. She has published more than 45 papers, about 20 of them in international journals.

Email: aabaraka62@googlemail.com