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World Biotechnology 2016
August 15-17, 2016
Volume 6 Issue 5(Suppl) J Biotechnol Biomater 2016
ISSN: 2155-952X JBTBM, an open access journal
conferenceseries .com
August 15-17, 2016 Sao Paulo, Brazil
Biotechnology World Convention
Juliana Ferreira de Santana et al., J Biotechnol Biomater 2016, 6:5(Suppl)
Linear peptides of Mycobacterium leprae antigens identified by SPOT synthesis indicate possible
targets for serum diagnosis of leprosy
Juliana Ferreira de Santana, Larissa M Alvarenga, Vanete Thomaz-Soccol and Juliana de Moura
Universidade Federal do Parana, Brazil
Leprosy is a chronic granulomatous infection that affects the skin, nasal mucosa and peripheral nerves caused by the Gram-
positive and obligate intracellular Bacillus, Mycobacterium leprae. The clinical manifestation of the infection with M.
leprae will depends on the immune condition of the host. To become the multidrug choice therapy easier, according to WHO,
the disease is divided into two categories: Paucibacillary and multibacillary leprosy. The paucibacillary patients present low
antibody titers and predominant cell-mediated immunity. Contrastingly, multibacillary patients have a cell-mediated immunity
inefficient with high antibody titers to M. leprae antigens. It will be interesting to identify and characterize biomarkers and
antigens for a nearly diagnosis of leprosy of both categories of patients. Thus, our strategy was to realize an epitope mapping of
seven proteins from M. leprae by using an array-based oligo-pepdide scanning (SPOT synthesis) onto a cellulose membrane
probed for reactivity with sera from leprosy patients. No protein has reactivity with sera from healthy volunteers while four
proteins have shown reactive spots when assayed with sera from leprosy patients. One of them was the 85B antigen from M.
leprae previously identified by our group as an immunodominant protein after being mimicked by conformational peptides
(mimotopes) by using Phage Display. After chemical synthesis, we hope the linear peptides found here could identify leprosy
patients by simple assays like as ELISA, independently of their categories.
Biography
Juliana Ferreira de Santana was graduated in Biotechnology at Universidade Federal de Uberlandia. She has published two scientific papers. Her Master in
Bioprocesses and Biotechnology Engineering was initiated in 2015 at Univesidade Federal de Parana.