euro-infectious-diseases-2016_posters-accepted-abstracts

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Volume 4, Issue 5(Suppl) J Infect Dis Ther
ISSN: 2332-0877 JIDT, an open access journal
Page 41
Euro Infectious Diseases 2016
September 05-06, 2016
conferenceseries.com
Infectious Diseases
September 05-06, 2016 Frankfurt, Germany
3rd Euro-Global Conference on
Detection of uncommon enteric bacterial pathogens from human diarrheal specimens by SYBR-Green
real time PCR
Goutam Chowdhury1, K Rajendran1, Asish K Mukhopadhyay 1 and Thandavarayan Ramamurthy1, 2
1National Institute of Cholera and Enteric Diseases, India
2Translational Health Science and Technology Institute, India
Acute diarrheal disease is still a major health problem and second most common cause of death worldwide in children under five
years of age. Most of the morbidity occurs in low-income countries, where the etiologies and epidemiology of gastroenteritis
remain incompletely understood. Diarrhea can be caused by a range of pathogens, including several bacteria. Conventional diagnostic
methods, such as culture, biochemical tests and enzyme-linked immunosorbent assay (ELISA) are laborious and time consuming.
We used SYBR-Green real time PCR assay targeting 10 uncommon diarrheagenic bacterial pathogens (S. aureus, Enterotoxigenic B.
cereus, C. perfringens, C. difficile, L. monocytogenes, P. shigelloides, Y. enterocolitica, Enterotoxigenic B. fragilis, A. hydrophila and P.
alcalifaciens) directly in fecal specimens from patients admitted infectious diseases hospital with acute diarrhea in Kolkata, India. The
products formed were identified based on melting point temperature (Tm) curve analysis. The assay was first validated with reference
strains or isolates and exhibited a limit of detection of 103 to 105 CFU/gm of stool for each pathogen. A total of 1184 clinical fecal
specimens from individual with diarrhea, previously cultured for enteric pathogens were evaluated. Enterotoxigenic B. fragilis was
detected highest number about 80 (6.75%) followed by enterotoxigenic B. cereus 60 (5.06%), C. perfringens 46 (3.88%), A. hydrophila
45 (3.80%), P. alcalifaciens 44 (3.71%), P. shigelloides 39 (3.29%), C. difficile 39 (3.29%), L. monocytogenes 38 (3.20%), S. aureus 23
(1.94%) and Y. enterocolitica 14 (1.8%) respectively. We found SYBR-Green real time PCR assay for simultaneous detection of 10
target pathogens to be comprehensive, rapid, inexpensive and accurate, of high selectivity and is well suited for surveillance or clinical
purpose.
J Infect Dis Ther 2016, 4:5(Suppl)
Comparison of virological and serological findings on Moroccan bluetongue virus 1 and 4 infected sheep
Kamar Drif
Institut Agronomique et Veterinaire Hassan II, Morocco
The bluetongue (BT) virus has been reported in Morocco in 2004. To investigate the involvement of BTV 1 and BTV4 infections,
on immunity of sheep and to provide a basis for interpretation of serological and virological data, experimental infections
were conducted with BTV-1 and BTV-4 strains. Antibody responses to BTV infections were evaluated using two enzyme linked
immunosorbent assays and microtiter serum neutralization tests (mSNTs) in addition to virological monitoring based on RT-PCR.
Large variation was observed between the three groups in clinical signs, showed variation in immune responses between animals.
Viremia for BT virus was readily detected in sheep following BTV-1 infection, but was not detected following exposure to BTV-4 in
group B and C. The high manifestation of clinical signs caused by BTV1 serotype compared to those caused by BTV4 could likely be
due to BTV strains antigenicity and could probably be responsible in suppressing or manifesting BT symptoms and viremia for this
serotype.