Journal of Stem Cell Research & Therapy

Jianjun Guan

Publications

• Research Article
  c-kit Positive Cardiac Outgrowth Cells Demonstrate Better Ability for Cardiac Recovery Against Ischemic Myopathy
  Author(s): Chuan Li, Satoshi Matsushita, Zhengqing Li, Jianjun Guan and Atsushi AmanoChuan Li, Satoshi Matsushita, Zhengqing Li, Jianjun Guan and Atsushi Amano
  
  Objective: Resident cardiac stem cells are expected to be a therapeutic option for patients who suffer from severe heart failure. However, uncertainty remains over whether sorting cells for c-kit, a stem cell marker, improves therapeutic outcomes. Materials and methods: Cardiac outgrowth cells cultured from explants of rat heart atrium were sorted according to their positivity (+) or negativity (-) for c-kit. These cells were exposed to hypoxia for 3 d, and subsequently harvested for mRNA expression measurement. The cell medium was also collected to assess cytokine secretion. To test for a functional benefit in animals, myocardial infarction (MI) was induced in rats, and c-kit+ or c-kit- cells were injected. The left ventricular ejection fraction (LVEF) was measured for up to 4 weeks, after which the heart was harvested for biological and hi.. View More»
  DOI: 10.4172/2157-7633.1000402

  Abstract PDF