3d-printing-2018-scientifictracks-abstracts

30 / 36

Page 75
Notes:
conferenceseries.com
Volume 9 International Journal of Advancements in Technology
ISSN: 0976-4860 3D Printing 2018
March 19-20, 2018
March 19-20, 2018 | London, UK
2nd International Conference on
3D Printing Technology and Innovations
Cell characterization methods for use in 3D bioprinting process development
Sarah Gretzinger1,2, Nicole Beckert2 and Jürgen Hubbuch1,2
1Institute of Functional Interfaces – KIT, Germany
2Karlsruhe Institute of Technology, Germany
The development of biocompatible 3D printing methods has pushed the limits in tissue engineering and regenerative
medicine in the past years and is considered to be a key technology in these application fields. Since the processing of
living materials represents a major increase in process complexity, a directed and systematic process development approach
is highly recommended for 3D bioprinting of cells. Such an approach is, however, profoundly dependent on the availability of
suitable and accurate cell characterization methods. In this study, we evaluated different state-of-the-art cell characterization
methods concerning applicability in 3D bioprinting process development. One metabolic assay, namely, PrestoBlue® and one
flow cytometry approach. The theoretical evaluation was based on method versatility and high-throughput screening (HTS)
compatibility, as well as method robustness. Further, we have evaluated the performance of two methods that differ in their
corresponding mechanism. In this case study, INS-1E was used as model cell line. The evaluation was done with one non-
invasive and one invasive cell characterisation method. As a non-invasive strategy, the metabolic assay PrestoBlue® was chosen,
since the colometric assay can be performed by analysing the supernatant. A flow cytometry strategy was chosen as an invasive
method. Here, a subsequent de-solubilization of the 3D printed object is necessary, in order to gain a single cell suspension.
Our study demonstrates the importance of analytical method evaluation, for a specific application, and will facilitate a guidance
for method selection.
Biography
Sarah Gretzinger is a PhD student at the Karlsruhe Institute of Technology, Karlsruhe, Germany. She has completed her Master’s studies form the University of
Ulm in cooperation with the Biberach University of Applied Science, Biberach an der Riss, Germany. Her research interest is: process development for cell-based
products.
Sarah Gretzinger et al., Int J Adv Technol 2018, Volume 9
DOI: 10.4172/0976-4860-C1-002